Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, 1 Haida South Road, Dinghai District, Zhoushan 316022, China.
Mar Drugs. 2020 Jul 18;18(7):370. doi: 10.3390/md18070370.
Collagen is a promising biomaterial used in the beauty and biomedical industries. In this study, the physicochemical characterization, antioxidant activities, and protective effects against HO-induced injury of collagen isolated from were investigated. The amino acid composition analysis showed that the collagen was rich in glycine (Gly), alanine (Ala), and glutamic acid (Glu), but poor in tyrosine (Tyr) and phenylalanine (Phe). Zeta potential analysis revealed that the isoelectric point (pI) of collagen from was about 4.25. It possessed moderate scavenging activities of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radicals in a dose-dependent manner. In addition, the collagen was able to effectively improve cell viability and morphology, inhibit the production of Malondialdehyde (MDA), and increase the activities of Superoxide Dismutase (SOD) and Glutathione Peroxidase (GSH-Px) in cultured RAW264.7 cells, resulting in a protective effect against HO-induced injury. Overall, the results showed that collagen extracted from has promising prospects in the beauty and cosmetics industries.
胶原蛋白是一种有前途的生物材料，广泛应用于美容和生物医学行业。在这项研究中，我们研究了从 中提取的胶原蛋白的物理化学特性、抗氧化活性以及对 HO 诱导损伤的保护作用。氨基酸组成分析表明，该胶原蛋白富含甘氨酸 (Gly)、丙氨酸 (Ala) 和谷氨酸 (Glu)，但酪氨酸 (Tyr) 和苯丙氨酸 (Phe) 含量较低。Zeta 电位分析表明， 胶原蛋白的等电点 (pI) 约为 4.25。它对 2,2-二苯基-1-苦基肼 (DPPH) 和 2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸 (ABTS) 自由基具有中等的清除活性，呈剂量依赖性。此外，该胶原蛋白能够有效提高细胞活力和形态，抑制丙二醛 (MDA) 的产生，增加培养的 RAW264.7 细胞中超氧化物歧化酶 (SOD) 和谷胱甘肽过氧化物酶 (GSH-Px) 的活性，对 HO 诱导的损伤具有保护作用。总的来说，这些结果表明，从 中提取的胶原蛋白在美容和化妆品行业具有广阔的应用前景。
