Department of Occupational Pneumology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Kitakyushu, Fukuoka, Japan.
Shared-Use Research Center, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Fukuoka, Japan.
J Occup Health. 2020 Jan;62(1):e12146. doi: 10.1002/1348-9585.12146.
Along with technological innovations for improving the efficiency of printing, nanoparticles have been added to the surface of toners, and there is concern about the harmful effects of those components. We investigated, through a long-term observation following intratracheal instillation using rats, whether exposure to a toner with external additives can cause tumorigenesis.
Female Wistar rats were intratracheally instilled with dispersed toner at low (1 mg/rat) and high (2 mg/rat) doses, and the rats were sacrificed at 24 months after exposure, after which we examined pulmonary inflammation, histopathological changes, and DNA damage in the lung. Rats that had deceased before 24 months were dissected at that time as well, to compare tumor development.
Although alveolar macrophages with pigment deposition in the alveoli were observed in the 1 and 2 mg exposure groups, no significant lung inflammation/fibrosis or tumor was observed. Since immunostaining with 8-OHdG or γ-H2AX did not show a remarkable positive reaction, it is thought that toner did not cause severe DNA damage to lung tissue.
These results suggest that toner with external additives may have low toxicity in the lung.
随着提高打印效率的技术创新，纳米颗粒已被添加到墨粉的表面，人们担心这些成分会产生有害影响。我们通过对大鼠气管内滴注进行长期观察，研究了暴露于含有外部添加剂的墨粉是否会导致肿瘤发生。
用分散的墨粉以低（1mg/大鼠）和高（2mg/大鼠）剂量对雌性 Wistar 大鼠进行气管内滴注，在暴露后 24 个月处死大鼠，然后检查肺部炎症、组织病理学变化和肺 DNA 损伤。在 24 个月之前死亡的大鼠也同时进行解剖，以比较肿瘤的发展情况。
尽管在 1 和 2mg 暴露组中观察到肺泡内有含色素的肺泡巨噬细胞，但没有观察到明显的肺部炎症/纤维化或肿瘤。由于 8-OHdG 或 γ-H2AX 的免疫染色没有显示出明显的阳性反应，因此认为墨粉没有对肺组织造成严重的 DNA 损伤。
这些结果表明，含有外部添加剂的墨粉在肺部的毒性可能较低。
