Kaplan Mahmut, Yilmaz Mustafa Mücahit, Say Rabia, Köprü Serap, Karaman Kevser
Erciyes University, Faculty of Agriculture, Field Crops Department, Kayseri, Turkey.
Dr. Yilmaz Medicinal Plants and Drug Raw Materials Company, Kayseri, Turkey.
Saudi J Biol Sci. 2020 Aug;27(8):1938-1946. doi: 10.1016/j.sjbs.2020.06.014. Epub 2020 Jun 16.
In the current study, the effect of glycerol -as a green solvent- addition into solvent mixture (50:50 ethanol:distilled water), on some biofunctional properties of L. was investigated. Response surface methodology (RSM) was used to detect the optimum conditions for the extraction process. Three variables namely glycerol concentration ( : 1-9 g), extraction temperature ( : 25-75 °C) and time ( :10-30 min) were selected and also total phenolic content, total flavonoid content, antioxidant capacity and antiradical activity of extracts were determined. Analysis of variance (ANOVA) showed that glycerol incorporation significantly increased the total phenolic content and antioxidant activity (p < 0.05) of the samples. Maximum levels to obtain the highest bioactive properties (highest total phenolic content and antioxidant activity) were determined as to be 9 g of glycerol addition for the extraction conditions as 45.4 °C and 75 min. This study reports the effect of glycerol on bioactive properties of and suggests that glycerol can be used to produce hydroalcoholic extracts having higher bioactivity from genus.
在本研究中，研究了作为绿色溶剂的甘油添加到溶剂混合物（50:50乙醇:蒸馏水）中对[具体植物名称未给出]某些生物功能特性的影响。采用响应面法（RSM）来检测提取过程的最佳条件。选择了三个变量，即甘油浓度（1 - 9克）、提取温度（25 - 75°C）和时间（10 - 30分钟），并测定了提取物的总酚含量、总黄酮含量、抗氧化能力和抗自由基活性。方差分析（ANOVA）表明，添加甘油显著提高了样品的总酚含量和抗氧化活性（p < 0.05）。在提取条件为45.4°C和75分钟时，获得最高生物活性特性（最高总酚含量和抗氧化活性）的甘油添加量最高为9克。本研究报道了甘油对[具体植物名称未给出]生物活性特性的影响，并表明甘油可用于从[具体植物属名未给出]属生产具有更高生物活性的水醇提取物。
