College of Materials Science and Technology, Qingdao University of Science and Technology, Qingdao, Shandong, 266042, China.
Department of Chemistry, University of Houston, Houston, TX, 77204, USA.
Small. 2020 Aug;16(34):e2001440. doi: 10.1002/smll.202001440. Epub 2020 Jul 26.
From manufacture to disposal, the interaction of graphdiyne based nanomaterials with living organisms is inevitable and crucial. However, the cytotoxic properties of this novel carbon nanomaterial are rarely investigated, and the mechanisms behind their cytotoxicity are totally unknown. In this study, the antibacterial activity of graphdiyne (GDY) and graphdiyne oxide (GDYO) is reported. GDY is capable of inhibiting broad-spectrum bacterial growth while exerting moderate cytotoxicity on mammalian cells. In comparison, GDYO exhibits lower antibacterial activity than that of GDY. Then an alterable, synergetic antibacterial mechanism of GDY, involving wrapping bacterial membrane, membrane insertion and disruption, and reactive oxygen species generation is demonstrated, while the differential gene expression analysis indicates that GDY could only alter the bacterial metabolism slightly and the oxidative stress route may be a minor bactericidal factor. The investigation of the antibacterial behaviors of GDY based nanomaterials may provide useful guidelines for the future design and application of this novel molecular allotrope of carbon.
从制造到处理，基于图形二炔的纳米材料与生物体的相互作用是不可避免的，也是至关重要的。然而，这种新型碳纳米材料的细胞毒性特性很少被研究，其细胞毒性的机制完全未知。在这项研究中，报告了图形二炔（GDY）和图形二炔氧化物（GDYO）的抗菌活性。GDY 能够抑制广谱细菌的生长，同时对哺乳动物细胞表现出适度的细胞毒性。相比之下，GDYO 的抗菌活性低于 GDY。然后，证明了 GDY 的一种可改变的、协同的抗菌机制，涉及包裹细菌膜、膜插入和破坏以及活性氧的产生，而差异基因表达分析表明，GDY 只能轻微改变细菌的代谢，氧化应激途径可能是一个较小的杀菌因素。对基于 GDY 的纳米材料的抗菌行为的研究可为这种新型碳同素异形体的未来设计和应用提供有用的指导。
