Division of Physical Biology, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, China.
University of Chinese Academy of Sciences, Beijing, China.
Cell Prolif. 2022 May;55(5):e13236. doi: 10.1111/cpr.13236. Epub 2022 May 3.
Silver nanoparticles (AgNPs) tend to aggregate spontaneously due to larger surface-to-volume ratio, which causes decreased antibacterial activity and even enhanced antimicrobial resistance (AMR). Here, we aim to improve the stability of AgNPs by employing a growth anchor graphdiyne (GDY) to overcome these shortcomings.
Bacillus subtilis and Escherichia coli were selected to represent gram-positive and gram-negative bacteria, respectively. Transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS), scanning electron microscopy (SEM)-EDS mapping and inductively coupled plasma mass spectrometry (ICP-MS) were carried out to characterize the physiochemical properties of materials. The antimicrobial property was determined by turbidimetry and plate colony-counting methods. The physiology of bacteria was detected by SEM and confocal imaging, such as morphology, reactive oxygen species (ROS) and cell membrane.
We successfully synthesized a hybrid graphdiyne @ silver nanoparticles (GDY@Ag) by an environment-friendly approach without any reductants. The hybrid showed high stability and excellent broad-spectrum antibacterial activity towards both gram-positive and gram-negative bacteria. It killed bacteria through membrane destruction and ROS production. Additionally, GDY@Ag did not induce the development of the bacterial resistance after repeated exposure.
GDY@Ag composite combats bacteria by synergetic action of GDY and AgNPs. Especially, GDY@Ag can preserve its bacterial susceptibility after repeated exposure compared to antibiotics. Our findings provide an avenue to design innovative antibacterial agents for effective sterilization.
由于较大的表面积与体积比，银纳米粒子（AgNPs）容易自发聚集，这导致其抗菌活性降低，甚至增强了抗菌耐药性（AMR）。在这里，我们旨在通过使用生长锚定的石墨炔（GDY）来提高 AgNPs 的稳定性，以克服这些缺点。
枯草芽孢杆菌和大肠杆菌分别被选作革兰氏阳性和革兰氏阴性细菌的代表。采用透射电子显微镜（TEM）、能谱（EDS）、扫描电子显微镜（SEM）-EDS 映射和电感耦合等离子体质谱（ICP-MS）对材料的理化性质进行了表征。采用浊度法和平板菌落计数法测定抗菌性能。通过 SEM 和共聚焦成像检测细菌的生理学，例如形态、活性氧（ROS）和细胞膜。
我们成功地通过一种无还原剂的环保方法合成了一种混合石墨炔@银纳米粒子（GDY@Ag）。该混合物表现出对革兰氏阳性和革兰氏阴性细菌的高稳定性和优异的广谱抗菌活性。它通过破坏细胞膜和产生 ROS 来杀死细菌。此外，GDY@Ag 在反复暴露后不会诱导细菌耐药性的发展。
GDY@Ag 复合材料通过 GDY 和 AgNPs 的协同作用来对抗细菌。特别是，与抗生素相比，GDY@Ag 在反复暴露后仍能保持其对细菌的敏感性。我们的研究结果为设计用于有效灭菌的创新型抗菌剂提供了一个途径。
