Department of Orthopaedics, Tianjin Medical University General Hospital, Tianjin, P. R. China.
International Science and Technology Cooperation Base of Spinal Cord Injury, Tianjin Key Laboratory of Spine and Spinal Cord, Tianjin Medical University General Hospital, Tianjin, P. R. China.
Gynecol Endocrinol. 2020 Nov;36(11):997-1001. doi: 10.1080/09513590.2020.1785420. Epub 2020 Jul 28.
MicroRNAs (miRNAs) represent RNA species found in serum. Many miRNAs were observed that were related to osteoporosis and osteopenia. However, expression and function analysis of miRNAs in postmenopausal osteoporosis (PMOP) remain unaddressed. We first compared the miRNA expression of blood samples in postmenopausal women with osteopenia or with osteoporosis via analysis of GSE64433. Bioinformatics analyses were conducted to get the key miRNAs and their functions and pathways. 331 miRNAs were being identified as differentially expressed miRNAs. Among these, 122 miRNA (36.86%) were up-regulated, and the remaining 209 miRNAs (63.14%) were down-regulated. 105 genes were predicted as the targets of these miRNAs. GO enrichment analysis results showed that the miRNAs mainly enriched in DNA binding, ATP binding, gene expression, regulation of the apoptotic process, chromatin binding, and protein kinase binding. KEGG enrichment analysis results demonstrated that the miRNAs mainly enriched in the TGF beta signaling pathway, wnt signaling pathway, JAK-STAT signaling pathway, and androgen receptor signaling pathway. This study identified the abundant differentially expressed miRNAs in the blood samples of postmenopausal women with osteopenia or with osteoporosis. This study may contribute to getting new diagnostic and therapeutic strategies for PMOP.
微小 RNA（miRNAs）是存在于血清中的 RNA 种类。许多与骨质疏松症和低骨量相关的 miRNA 被观察到。然而，绝经后骨质疏松症（PMOP）中 miRNA 的表达和功能分析仍未得到解决。我们首先通过分析 GSE64433 比较了骨质疏松症和低骨量绝经后妇女血液样本中的 miRNA 表达。进行了生物信息学分析以获得关键 miRNA 及其功能和途径。确定了 331 个差异表达 miRNA。其中，122 个 miRNA（36.86%）上调，其余 209 个 miRNA（63.14%）下调。预测这些 miRNA 的 105 个靶基因。GO 富集分析结果表明，miRNA 主要富集在 DNA 结合、ATP 结合、基因表达、凋亡过程调控、染色质结合和蛋白激酶结合。KEGG 富集分析结果表明，miRNA 主要富集在 TGF-β 信号通路、Wnt 信号通路、JAK-STAT 信号通路和雄激素受体信号通路。本研究鉴定了绝经后妇女骨质疏松症和低骨量血液样本中丰富的差异表达 miRNA。这一研究可能有助于为 PMOP 获得新的诊断和治疗策略。
