Department of Medical Biology, Faculty of Medicine, SANKO University, Gaziantep, Turkey
Department of Biophysics, Faculty of Medicine, SANKO University, Gaziantep, Turkey.
Biosci Rep. 2019 May 14;39(5). doi: 10.1042/BSR20190667. Print 2019 May 31.
MicroRNAs (miRNAs) have been proven to play a crucial role in postmenopausal osteoporosis (PMO), and studies on their diagnostic value have been increasing. In our study, we aim to identify the key miRNAs in the PMO that might be potential biomarkers. A comprehensive systematic literature search was conducted by searching PubMed, Web of Science, Embase and Cochrane Library databases. In the total of 16 independent miRNA expression studies which contained 327 PMO patients and 328 postmenopausal (PM) healthy control samples, miRNAs were evaluated by using robust rank aggregation (RRA) method. A statistically significant meta-signature of up-regulated hsa-miR-133a-3p ( = 1.38e-03) was determined. Then bioinformatics analysis to recruit putative target genes prediction of hsa-miR-133a-3p and pathway enrichment analysis to reveal what biological processes this miRNA may affect were conducted. It was indicated that pathways were commonly associated with adrenergic signaling in cardiomyocytes, adherens junction, PI3K-Akt signaling pathway and AMPK signaling pathway. Furthermore, STRING and Cytoscape tools were used to visualize the interactions between target genes of hsa-miR-133a-3p. Six genes were detected as hub genes among 576 targets which were CDC42, RHOA, EGFR, VAMP2, PIK3R2 and FN1. After Kyoto Encyclopedia of Genes and Genomes pathway analysis, it was detected that these hub genes were mostly enriched in signaling pathways and cancer. In this meta-analysis, it is stated that circulating hsa-miR-133a-3p may serve as a potential non-invasive biomarker and therapeutic target in PMO.
微小 RNA（miRNAs）已被证明在绝经后骨质疏松症（PMO）中发挥着关键作用，并且对其诊断价值的研究也在不断增加。在我们的研究中，我们旨在确定 PMO 中可能成为潜在生物标志物的关键 miRNAs。通过搜索 PubMed、Web of Science、Embase 和 Cochrane Library 数据库进行了全面的系统文献检索。在总共 16 项独立的 miRNA 表达研究中，包含了 327 名 PMO 患者和 328 名绝经后（PM）健康对照组样本，使用稳健秩聚合（RRA）方法评估了 miRNAs。确定了一个统计学上显著的上调 hsa-miR-133a-3p 的元特征（ = 1.38e-03）。然后进行了生物信息学分析，以招募 hsa-miR-133a-3p 的推测靶基因预测，并进行了途径富集分析，以揭示该 miRNA 可能影响的生物学过程。结果表明，途径通常与心肌细胞中的肾上腺素能信号、黏着连接、PI3K-Akt 信号通路和 AMPK 信号通路相关。此外，使用 STRING 和 Cytoscape 工具可视化了 hsa-miR-133a-3p 的靶基因之间的相互作用。在 576 个靶基因中检测到六个基因（CDC42、RHOA、EGFR、VAMP2、PIK3R2 和 FN1）作为 hub 基因。经过京都基因与基因组百科全书途径分析，发现这些 hub 基因主要富集在信号通路和癌症中。在这项荟萃分析中，表明循环 hsa-miR-133a-3p 可能作为 PMO 的一种潜在非侵入性生物标志物和治疗靶点。
