prevents Heidelberg translocation in co-culture model mimicking intestinal epithelium.

Heidelberg is one of the most common serovar causing foodborne illnesses. To limit the development of digestive bacterial infection, food supplements containing probiotic bacteria can be proposed. Commensal non-toxigenic has recently been suggested as a next-generation probiotic candidate. By using an original triple co-culture model including Caco-2 cells (representing human enterocytes), HT29-MTX (representing mucus-secreting goblet cells), and M cells differentiated from Caco-2 by addition of Raji B lymphocytes, bacterial translocation was evaluated. The data showed that Heidelberg could translocate in the triple co-culture model with high efficiency, whereas for a weak translocation was obtained. When cells were exposed to both bacteria, Heidelberg translocation was inhibited. The cell-free supernatant of also inhibited Heidelberg translocation without impacting epithelial barrier integrity. This supernatant did not affect the growth of Heidelberg. The non-toxigenic confers health benefits to the host by reducting bacterial translocation. These results suggested that the multicellular model provides an efficient model to evaluate the translocation of pathogens and to screen for probiotics that have a potential inhibitory effect on this translocation.