• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

miR-28-5p 介导姜黄素对人弥漫大 B 细胞淋巴瘤细胞的抗增殖和促凋亡作用。

MiR-28-5p mediates the anti-proliferative and pro-apoptotic effects of curcumin on human diffuse large B-cell lymphoma cells.

机构信息

Department of Pediatrics, People's Hospital of Shijiazhuang City, Shijiazhuang, China.

Department of Rehabilitation, The Second Hospital of Hebei Medical University, Shijiazhuang, China.

出版信息

J Int Med Res. 2020 Jul;48(7):300060520943792. doi: 10.1177/0300060520943792.

DOI:10.1177/0300060520943792
PMID:32721183
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7388109/
Abstract

OBJECTIVE

To investigate the anti-proliferative and pro-apoptotic effects of curcumin on diffuse large B-cell lymphoma (DLBCL) cells and explore the mechanism.

METHODS

OCI-LY7 cells were treated with curcumin (2.5, 5, 10, 20, and 40 μM) for 24, 48, or 72 hours. Cell viability and apoptosis were determined using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5 diphenyl tetrazolium bromide assay and TdT-mediated dUTP nick-end labeling staining, respectively. MiR-28-5p expression was detected via qRT-PCR. The binding site of miR-28-5p was predicted using online databases and verified using the dual-luciferase reporter assay. MiR-28-5p overexpression and inhibition were achieved via transfection with an miR-28-5p mimic and inhibitor, respectively.

RESULTS

Curcumin decreased the viability of OCI-LY7 cells in a concentration- and time-dependent manner, and these effects were attenuated by miR-28-5p inhibition. MiR-28-5p expression was upregulated by curcumin. Curcumin increased the numbers of apoptotic cells and upregulated cleaved caspase-3 expression, and these effects were attenuated by miR-28-5p inhibition. The dual-luciferase reporter assay confirmed that miR-28-5p directly targets the 3'-untranslated region of BECN1. Curcumin downregulated BECN1 and microtubule-associated protein 1 light chain 3 beta-II/I expression and upregulated p62 expression.

CONCLUSIONS

Our results described the curcumin exerted anti-proliferative and pro-apoptotic effects on OCI-LY7 cells through a mechanism potentially involving miR-28-5p.

摘要

目的

研究姜黄素对弥漫性大 B 细胞淋巴瘤（DLBCL）细胞的抗增殖和促凋亡作用，并探讨其机制。

方法

用姜黄素（2.5、5、10、20 和 40 μM）处理 OCI-LY7 细胞 24、48 或 72 小时。用 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）比色法和末端转移酶介导的 dUTP 缺口末端标记染色法分别检测细胞活力和细胞凋亡。用 qRT-PCR 检测 miR-28-5p 的表达。利用在线数据库预测 miR-28-5p 的结合位点，并通过双荧光素酶报告基因检测进行验证。通过转染 miR-28-5p 模拟物和抑制剂分别实现 miR-28-5p 的过表达和抑制。

结果

姜黄素呈浓度和时间依赖性地降低 OCI-LY7 细胞的活力，而 miR-28-5p 抑制则减弱了这些作用。姜黄素上调 miR-28-5p 的表达。姜黄素增加凋亡细胞数量并上调 cleaved caspase-3 表达，而 miR-28-5p 抑制则减弱了这些作用。双荧光素酶报告基因检测证实 miR-28-5p 可直接靶向 BECN1 的 3'-非翻译区。姜黄素下调 BECN1 和微管相关蛋白 1 轻链 3β-II/I 的表达，并上调 p62 的表达。

结论

我们的结果描述了姜黄素通过一种可能涉及 miR-28-5p 的机制对 OCI-LY7 细胞发挥抗增殖和促凋亡作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2fa/7388109/e5637868c978/10.1177_0300060520943792-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2fa/7388109/abf23a29c25d/10.1177_0300060520943792-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2fa/7388109/cd01a9ba7cc3/10.1177_0300060520943792-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2fa/7388109/f083114e9b34/10.1177_0300060520943792-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2fa/7388109/babf9b23d596/10.1177_0300060520943792-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2fa/7388109/1d3325e97f50/10.1177_0300060520943792-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2fa/7388109/e5637868c978/10.1177_0300060520943792-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2fa/7388109/abf23a29c25d/10.1177_0300060520943792-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2fa/7388109/cd01a9ba7cc3/10.1177_0300060520943792-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2fa/7388109/f083114e9b34/10.1177_0300060520943792-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2fa/7388109/babf9b23d596/10.1177_0300060520943792-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2fa/7388109/1d3325e97f50/10.1177_0300060520943792-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2fa/7388109/e5637868c978/10.1177_0300060520943792-fig6.jpg

相似文献

1
MiR-28-5p mediates the anti-proliferative and pro-apoptotic effects of curcumin on human diffuse large B-cell lymphoma cells.miR-28-5p 介导姜黄素对人弥漫大 B 细胞淋巴瘤细胞的抗增殖和促凋亡作用。
J Int Med Res. 2020 Jul;48(7):300060520943792. doi: 10.1177/0300060520943792.
2
Curcumin Inhibits the Proliferation, Migration, Invasion, and Apoptosis of Diffuse Large B-Cell Lymphoma Cell Line by Regulating MiR-21/VHL Axis.姜黄素通过调控 miR-21/VHL 轴抑制弥漫大 B 细胞淋巴瘤细胞系的增殖、迁移、侵袭和凋亡。
Yonsei Med J. 2020 Jan;61(1):20-29. doi: 10.3349/ymj.2020.61.1.20.
3
HOXD3 Up-regulating KDM5C Promotes Malignant Progression of Diffuse Large B-Cell Lymphoma by Decreasing p53 Expression.HOXD3 通过降低 p53 表达促进弥漫性大 B 细胞淋巴瘤的恶性进展。
Balkan Med J. 2022 Jan 25;39(1):30-38. doi: 10.5152/balkanmedj.2021.21068. Epub 2021 Dec 20.
4
Geniposide inhibits proliferation and induces apoptosis of diffuse large B-cell lymphoma cells by inactivating the HCP5/miR-27b-3p/MET axis.栀子苷通过失活 HCP5/miR-27b-3p/MET 轴抑制弥漫性大 B 细胞淋巴瘤细胞的增殖并诱导其凋亡。
Int J Med Sci. 2020 Sep 23;17(17):2735-2743. doi: 10.7150/ijms.51329. eCollection 2020.
5
CircRNA_OTUD7A upregulates FOXP1 expression to facilitate the progression of diffuse large B-cell lymphoma via acting as a sponge of miR-431-5p.环状RNA_OTUD7A通过充当miR-431-5p的海绵上调FOXP1表达，以促进弥漫性大B细胞淋巴瘤的进展。
Genes Genomics. 2021 Jun;43(6):653-667. doi: 10.1007/s13258-021-01094-z. Epub 2021 Apr 8.
6
Long non-coding RNA SNHG16 promotes proliferation and inhibits apoptosis of diffuse large B-cell lymphoma cells by targeting miR-497-5p/PIM1 axis.长链非编码 RNA SNHG16 通过靶向 miR-497-5p/PIM1 轴促进弥漫性大 B 细胞淋巴瘤细胞的增殖并抑制其凋亡。
J Cell Mol Med. 2019 Nov;23(11):7395-7405. doi: 10.1111/jcmm.14601. Epub 2019 Sep 4.
7
MiR-214 prevents the progression of diffuse large B-cell lymphoma by targeting PD-L1.miR-214 通过靶向 PD-L1 抑制弥漫大 B 细胞淋巴瘤的进展。
Cell Mol Biol Lett. 2019 Dec 4;24:68. doi: 10.1186/s11658-019-0190-9. eCollection 2019.
8
[Curcumin targeting miR-155-5p/TP53INP1 axis induced oxidative stress to regulate salivary gland tumor cell proliferation and apoptosis].[姜黄素靶向miR-155-5p/TP53INP1轴诱导氧化应激以调节涎腺肿瘤细胞增殖和凋亡]
Shanghai Kou Qiang Yi Xue. 2022 Oct;31(5):483-490.
9
LncRNA PVT1 facilitates DLBCL development via miR-34b-5p/Foxp1 pathway.长链非编码 RNA PVT1 通过 miR-34b-5p/Foxp1 通路促进弥漫性大 B 细胞淋巴瘤的发展。
Mol Cell Biochem. 2022 Mar;477(3):951-963. doi: 10.1007/s11010-021-04335-7. Epub 2022 Jan 31.
10
Curcumin Increases Radiosensitivity of Radioresistant Nasopharyngeal Cancer.姜黄素增加耐辐射鼻咽癌的放射敏感性。
Discov Med. 2023 Jun;35(176):418-428. doi: 10.24976/Discov.Med.202335176.42.

引用本文的文献

1
miR-28: A Tiny Player in Cancer Progression and Other Human Diseases.微小RNA-28：癌症进展及其他人类疾病中的小角色
Biomolecules. 2025 May 24;15(6):757. doi: 10.3390/biom15060757.
2
Ferroptosis-Regulated Natural Products and miRNAs and Their Potential Targeting to Ferroptosis and Exosome Biogenesis.铁死亡调控天然产物和 miRNAs 及其对铁死亡和外泌体生物发生的潜在靶向作用。
Int J Mol Sci. 2024 May 31;25(11):6083. doi: 10.3390/ijms25116083.
3
Curcumin in treatment of hematological cancers: Promises and challenges.姜黄素治疗血液系统癌症：前景与挑战

本文引用的文献

1
Strategy for improved therapeutic efficiency of curcumin in the treatment of gastric cancer.提高姜黄素治疗胃癌疗效的策略。
Biomed Pharmacother. 2019 Oct;118:109278. doi: 10.1016/j.biopha.2019.109278. Epub 2019 Aug 3.
2
Mechanism of Apoptosis Induced by Curcumin in Colorectal Cancer.姜黄素诱导结直肠癌细胞凋亡的机制。
Int J Mol Sci. 2019 May 17;20(10):2454. doi: 10.3390/ijms20102454.
3
The molecular machinery of regulated cell death.调控细胞死亡的分子机制。
J Tradit Complement Med. 2024 Jan 5;14(2):121-134. doi: 10.1016/j.jtcme.2023.10.004. eCollection 2024 Mar.
4
Contribution of Non-Coding RNAs to Anticancer Effects of Dietary Polyphenols: Chlorogenic Acid, Curcumin, Epigallocatechin-3-Gallate, Genistein, Quercetin and Resveratrol.非编码RNA在膳食多酚抗癌作用中的贡献：绿原酸、姜黄素、表没食子儿茶素-3-没食子酸酯、染料木黄酮、槲皮素和白藜芦醇。
Antioxidants (Basel). 2022 Nov 28;11(12):2352. doi: 10.3390/antiox11122352.
5
Using ncRNAs as Tools in Cancer Diagnosis and Treatment-The Way towards Personalized Medicine to Improve Patients' Health.利用 ncRNAs 作为癌症诊断和治疗的工具——迈向改善患者健康的个体化医疗之路。
Int J Mol Sci. 2022 Aug 19;23(16):9353. doi: 10.3390/ijms23169353.
6
Effects of hsa-miR-28-5p on Adriamycin Sensitivity in Diffuse Large B-Cell Lymphoma.人源微小RNA-28-5p对弥漫性大B细胞淋巴瘤阿霉素敏感性的影响
Evid Based Complement Alternat Med. 2022 Jul 13;2022:4290994. doi: 10.1155/2022/4290994. eCollection 2022.
7
The emerging role non-coding RNAs in B cell-related disorders.非编码RNA在B细胞相关疾病中的新兴作用。
Cancer Cell Int. 2022 Feb 22;22(1):91. doi: 10.1186/s12935-022-02521-1.
Cell Res. 2019 May;29(5):347-364. doi: 10.1038/s41422-019-0164-5. Epub 2019 Apr 4.
4
Autophagy: Supporting cellular and organismal homeostasis by self-eating.自噬：通过自我吞噬来支持细胞和机体的内稳态。
Int J Biochem Cell Biol. 2019 Jun;111:1-10. doi: 10.1016/j.biocel.2019.03.010. Epub 2019 Mar 30.
5
Systematic Review of the Potential of MicroRNAs in Diffuse Large B Cell Lymphoma.微小RNA在弥漫性大B细胞淋巴瘤中作用潜力的系统评价
Cancers (Basel). 2019 Jan 26;11(2):144. doi: 10.3390/cancers11020144.
6
Antitumor Effect of Various Phytochemicals on Diverse Types of Thyroid Cancers.各种植物化学物质对不同类型甲状腺癌的抗肿瘤作用。
Nutrients. 2019 Jan 9;11(1):125. doi: 10.3390/nu11010125.
7
Curcumin as a therapeutic agent in leukemia.姜黄素在白血病治疗中的应用。
J Cell Physiol. 2019 Aug;234(8):12404-12414. doi: 10.1002/jcp.28072. Epub 2019 Jan 4.
8
MicroRNAs as Biomarkers of B-cell Lymphoma.微小RNA作为B细胞淋巴瘤的生物标志物
Biomark Insights. 2018 Oct 16;13:1177271918806840. doi: 10.1177/1177271918806840. eCollection 2018.
9
Circulating miRNAs as biomarkers in diffuse large B-cell lymphoma: a systematic review.循环微小RNA作为弥漫性大B细胞淋巴瘤的生物标志物：一项系统综述
Oncotarget. 2018 Apr 27;9(32):22850-22861. doi: 10.18632/oncotarget.25230.
10
The Function of MicroRNAs in B-Cell Development, Lymphoma, and Their Potential in Clinical Practice.MicroRNAs 在 B 细胞发育、淋巴瘤中的作用及其在临床实践中的潜力。
Front Immunol. 2018 Apr 30;9:936. doi: 10.3389/fimmu.2018.00936. eCollection 2018.