Luskin M B, Pearlman A L, Sanes J R
Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, Missouri 63110.
Neuron. 1988 Oct;1(8):635-47. doi: 10.1016/0896-6273(88)90163-8.
To analyze cell lineage in the murine cerebral cortex, we infected progenitor cells with a recombinant retrovirus, then used the retroviral gene product to identify the descendants of infected cells. Cortices were infected on E12-E14 either in vivo or following dissociation and culture. In both cases, nearly all clones contained either neurons or glia, but not both. Thus, neuronal and glial lineages appear to diverge early in cortical development. To analyze the distribution of clonally related cells in vivo, clonal boundaries were reconstructed from serial sections. Perinatally (E18-PN0), clonally related cells were radially arrayed as they migrated to the cortical plate. Thus, clonal cohorts traverse a similar radial path. Following migration (PN7-PN23), neuronal clones generally remained radially arrayed, while glial clones were variable in orientation, suggesting that these two cell types accumulate in different ways. Neuronal clones sometimes spanned the full thickness of the cortex. Thus, a single progenitor can contribute neurons to several laminae.
为了分析小鼠大脑皮层中的细胞谱系，我们用重组逆转录病毒感染祖细胞，然后利用逆转录病毒基因产物来识别受感染细胞的后代。在胚胎第12 - 14天，通过体内感染或解离培养后感染皮层。在这两种情况下，几乎所有的克隆都只包含神经元或神经胶质细胞，而非两者皆有。因此，神经元和神经胶质细胞谱系似乎在皮层发育早期就发生了分化。为了分析体内克隆相关细胞的分布，从连续切片重建克隆边界。围产期（胚胎第18天至出生后第0天），克隆相关细胞在迁移到皮层板时呈放射状排列。因此，克隆群体沿着相似的放射状路径迁移。迁移后（出生后第7天至第23天），神经元克隆通常仍呈放射状排列，而神经胶质细胞克隆的方向则各不相同，这表明这两种细胞类型以不同的方式聚集。神经元克隆有时跨越皮层的全层。因此，单个祖细胞可以为多个层提供神经元。
