Mun Ha In, Kim Yangmin X, Suh Dong Ho, Lee Seulbi, Singh Digar, Jung Eun Sung, Lee Choong Hwan, Sung Jwakyung
Department of Bioscience and Biotechnology, Konkuk University, Seoul, Korea.
National Institute of Agricultural Sciences, Rural Development Administration, Wanju, Republic of Korea.
PLoS One. 2020 Jul 29;15(7):e0236813. doi: 10.1371/journal.pone.0236813. eCollection 2020.
High salt accumulation, resulting from the rampant use of chemical fertilizers in greenhouse cultivation, has deleterious effects on plant growth and crop yield. Herein, we delineated the effects of magnesium (Mg) oversupply on Perilla frutescens leaves, a traditional edible and medicinal herb used in East-Asian countries. Mg oversupply resulted in significantly higher chlorophyll content coupled with lower antioxidant activities and growth, suggesting a direct effect on subtle metabolomes. The relative abundance of bioactive phytochemicals, such as triterpenoids, flavonoids, and cinnamic acids, was lower in the Mg-oversupplied plants than in the control. Correlation analysis between plant phenotypes (plant height, total fresh weight of the shoot, leaf chlorophyll content, and leaf antioxidant content) and the altered metabolomes in P. frutescens leaves suggested an acclimatization mechanism to Mg oversupply. In conclusion, P. frutescens preferentially accumulated compatible solutes, i.e., carbohydrates and amino acids, to cope with higher environmental Mg levels, instead of employing secondary and antioxidative metabolism.
由于温室种植中化肥的大量使用导致高盐积累，这对植物生长和作物产量产生有害影响。在此，我们描述了镁（Mg）过量供应对紫苏叶的影响，紫苏是东亚国家使用的一种传统食用和药用草本植物。镁过量供应导致叶绿素含量显著升高，同时抗氧化活性和生长降低，表明对微妙的代谢组有直接影响。在镁过量供应的植物中，三萜类、黄酮类和肉桂酸等生物活性植物化学物质的相对丰度低于对照。紫苏叶的植物表型（株高、地上部总鲜重、叶片叶绿素含量和叶片抗氧化剂含量）与代谢组变化之间的相关性分析表明存在对镁过量供应的适应机制。总之，紫苏优先积累相容性溶质，即碳水化合物和氨基酸，以应对较高的环境镁水平，而不是采用次生代谢和抗氧化代谢。
