Biotechnology Department, University of Verona, Strada Le Grazie 15, 37134, Verona, Italy.
Department for Sustainable Food Process, Università Cattolica del Sacro Cuore, via Emilia Parmense 84, Piacenza, Italy.
BMC Plant Biol. 2019 Apr 16;19(1):148. doi: 10.1186/s12870-019-1726-x.
Magnesium (Mg) deficiency causes physiological and molecular responses, already dissected in several plant species. The study of these responses among genotypes showing a different tolerance to the Mg shortage can allow identifying the mechanisms underlying the resistance to this nutritional disorder. To this aim, we compared the physiological and molecular responses (e.g. changes in root metabolome and transcriptome) of two grapevine rootstocks exhibiting, in field, different behaviors with respect to Mg shortage (1103P, tolerant and SO4 susceptible).
The two grapevine rootstocks confirmed, in a controlled growing system, their behavior in relation to the tolerance to Mg deficiency. Differences in metabolite and transcriptional profiles between the roots of the two genotypes were mainly linked to antioxidative compounds and the cell wall constituents. In addition, differences in secondary metabolism, in term of both metabolites (e.g. alkaloids, terpenoids and phenylpropanoids) and transcripts, assessed between 1103P and SO4 suggest a different behavior in relation to stress responses particularly at early stages of Mg deficiency.
Our results suggested that the higher ability of 1103P to tolerate Mg shortage is mainly linked to its capability of coping, faster and more efficiently, with the oxidative stress condition caused by the nutritional disorder.
镁（Mg）缺乏会引起生理和分子反应，这些反应已在几种植物物种中得到解析。在对镁缺乏具有不同耐受能力的基因型中研究这些反应，可以确定对这种营养障碍产生抗性的机制。为此，我们比较了两种表现出不同耐镁缺素能力的葡萄砧木的生理和分子反应（例如，根系代谢组和转录组的变化）（1103P，耐缺素和 SO4 敏感）。
在受控生长系统中，两种葡萄砧木证实了其与镁缺乏耐受性相关的行为。两种基因型根系之间的代谢物和转录物谱差异主要与抗氧化化合物和细胞壁成分有关。此外，1103P 和 SO4 之间次生代谢物（例如生物碱、萜类和苯丙素类）和转录物的差异表明，在镁缺乏的早期阶段，它们在应激反应方面的表现不同。
我们的结果表明，1103P 更高的耐镁缺乏能力主要与其更快、更有效地应对营养障碍引起的氧化应激条件的能力有关。
