Wang Wei, Shu Mingming, Li Jianhua, Wang Qihang, Zhang Wendan, Wang Ye, Guo Yiming, Cheng Yanbin, Jiang Honghong, Song Chunlan, Liu Yuan, Shang Wei
Department of Obstetrics and Gynecology, The Seventh Medical Center of Chinese People's Liberation Army (PLA) General Hospital, Beijing, China.
Department of Obstetrics and Gynecology, Chinese PLA General Hospital, Beijing, China.
Front Endocrinol (Lausanne). 2025 Jan 7;15:1447397. doi: 10.3389/fendo.2024.1447397. eCollection 2024.
Premature ovarian insufficiency (POI) is a condition characterized by ovarian dysfunction occurring before the age of 40, and its etiology is multifactorial, including genetic, immunological, infectious, environmental, and iatrogenic factors, with over half of the cases remaining unexplained. Whether the microbial communities and metabolites in follicular fluid, which is the direct microenvironment for oocyte survival, are related to POI has not been reported.
In this study, Follicular fluid samples of 26 patients with POI and 27 controls with a normal ovarian reserve were collected and analyzed using 16S rDNA sequencing and untargeted metabolomics. Conjoint analysis was performed to identify key microbial communities and metabolites that might be involved in POI.
Patients with POI exhibited significant alterations in microbial richness and diversity and metabolic profile in their follicular fluid. The downregulation of ABC transporters and upregulation of the citrate cycle (TCA cycle) might be critical for the development and progression of POI. G-Rhodopseudomonas and g-Caulobacter were identified as key microbial genera, while L-aspartic acid, citrate, isoleucine, and cytidine were identified as key metabolites.
These findings offer novel insights into the pathogenesis of POI and might pave the way for improved clinical outcomes for individuals with POI.
卵巢早衰（POI）是一种以40岁之前出现卵巢功能障碍为特征的病症，其病因是多因素的，包括遗传、免疫、感染、环境和医源性因素，超过一半的病例病因不明。卵泡液是卵母细胞存活的直接微环境，其中的微生物群落和代谢产物是否与POI相关尚未见报道。
在本研究中，收集了26例POI患者和27例卵巢储备功能正常的对照者的卵泡液样本，并使用16S rDNA测序和非靶向代谢组学进行分析。进行联合分析以鉴定可能参与POI的关键微生物群落和代谢产物。
POI患者的卵泡液中微生物丰富度、多样性和代谢谱存在显著改变。ABC转运蛋白的下调和柠檬酸循环（TCA循环）的上调可能对POI的发生和发展至关重要。G-红假单胞菌属和g-柄杆菌属被鉴定为关键微生物属，而L-天冬氨酸、柠檬酸、异亮氨酸和胞苷被鉴定为关键代谢产物。
这些发现为POI的发病机制提供了新的见解，并可能为改善POI患者的临床结局铺平道路。
