Springer M, Graffe M, Grunberg-Manago M
Mol Gen Genet. 1979 Feb 1;169(3):337-43. doi: 10.1007/BF00382279.
A set of lambda transducing phages carrying varying lengths of the E. coli chromosome around the structural gene for initiation factor IF3 (infC) was derived from lambda p2 which is known to carry, besides infC, the structural genes for the alpha subunit of phenylalanyl-tRNA synthetase (pheS), the beta subunit of phenylalanyl-tRNA synthetase (pheT) and the structural gene for threonyl-tRNA synthetase (thrS). The E. coli coding content of these derived phages was analysed by genetic complementation of a set of mutants and by SDS-polyacrylamide gel analysis of the proteins synthesized in UV irradiated cells infected with these phages. The segregation pattern of the different genes among these derived phages indicates that the order of the genes is pheT - pheS - "P12" - (infC, thrS) where infC is probably between "P12" and thrS. "P12" is the structural gene of a 12,000 molecular weight unidentified protein.
一组携带大肠杆菌染色体围绕起始因子IF3（infC）结构基因不同长度片段的λ转导噬菌体，是从λp2衍生而来的。已知λp2除了携带infC外，还携带苯丙氨酰 - tRNA合成酶α亚基（pheS）、苯丙氨酰 - tRNA合成酶β亚基（pheT）的结构基因以及苏氨酰 - tRNA合成酶（thrS）的结构基因。通过一组突变体的遗传互补以及对感染这些噬菌体的紫外线照射细胞中合成的蛋白质进行SDS - 聚丙烯酰胺凝胶分析，对这些衍生噬菌体的大肠杆菌编码内容进行了分析。这些衍生噬菌体中不同基因的分离模式表明，基因顺序为pheT - pheS - “P12” - （infC，thrS），其中infC可能位于“P12”和thrS之间。“P12”是一种分子量为12,000的未鉴定蛋白质的结构基因。
