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鱼类腐败细菌、鱼类病原体及相关物种的胞外核酸酶活性。

Extracellular nuclease activity of fish spoilage bacteria, fish pathogens, and related species.

作者信息

Sadovski A Y, Levin R E

出版信息

Appl Microbiol. 1969 Jun;17(6):787-9. doi: 10.1128/am.17.6.787-789.1969.

DOI:10.1128/am.17.6.787-789.1969
PMID:5797936
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC377811/
Abstract

The production of extracellular deoxyribonuclease and ribonuclease by 23 marine and 3 dairy strains of Pseudomonas putrefaciens, 15 strains of fish-pathogenic fluorescent pseudomonads, 38 strains of fluorescent pseudomonads isolated from haddock, and 34 related organisms was determined by an agar plate method. All strains of P. putrefaciens produced both deoxyribonuclease and ribonuclease. Of the other 87 organisms examined, 26.5% produced ribonuclease and 14.5% produced deoxyribonuclease. All organisms which produced deoxyribonuclease also produced ribonuclease. Deoxyribonuclease production by P. putrefaciens is suggested as a useful criterion of identity for members of this intense fish spoilage species.

摘要

采用琼脂平板法测定了23株海洋腐败假单胞菌、3株乳源腐败假单胞菌、15株鱼致病性荧光假单胞菌、38株从黑线鳕中分离出的荧光假单胞菌以及34株相关微生物产生细胞外脱氧核糖核酸酶和核糖核酸酶的情况。所有腐败假单胞菌菌株均能产生脱氧核糖核酸酶和核糖核酸酶。在检测的其他87种微生物中,26.5%能产生核糖核酸酶,14.5%能产生脱氧核糖核酸酶。所有产生脱氧核糖核酸酶的微生物也都能产生核糖核酸酶。建议将腐败假单胞菌产生脱氧核糖核酸酶作为该严重致鱼腐败菌种成员鉴定的一个有用标准。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d952/377811/b8ea95f4e74a/applmicro00006-0025-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d952/377811/b8ea95f4e74a/applmicro00006-0025-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d952/377811/b8ea95f4e74a/applmicro00006-0025-a.jpg

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本文引用的文献

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Extracellular Deoxyribonucleases in Members of the Family Enterobacteriaceae.肠杆菌科成员中的细胞外脱氧核糖核酸酶
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来自食品、人类临床标本及其他来源的腐败假单胞菌分离株的DNA碱基组成与代谢的相关性
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Characterization of some fish and shrimp spoiling bacteria.一些鱼类和虾类腐败细菌的特性分析
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Measurement of hypoxanthine concentration in canned herring as an index of the freshness of the raw material, with a comment on flavour relations.测定罐装鲱鱼中次黄嘌呤的浓度作为原料新鲜度的指标,并对风味关系进行评论。
J Sci Food Agric. 1966 Sep;17(9):434-6. doi: 10.1002/jsfa.2740170911.
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Use of the deoxyribonuclease test as an aid in the differentiation of Paracolobactrum (Hafnia) from serratia.使用脱氧核糖核酸酶试验辅助鉴别副结肠杆菌(哈夫尼亚菌属)和沙雷菌属。
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