Tiradentes University (UNIT), Aracaju/SE, Brazil.
Federal University of Amazonas (UFAM), Itacoatiara/AM, Brazil.
Nanomedicine. 2020 Oct;29:102272. doi: 10.1016/j.nano.2020.102272. Epub 2020 Jul 27.
Carbon nanotubes (CN) have been studied to treat spinal cord injuries because of its electrical properties and nanometric dimensions. This work aims to develop a photopolymerizable hydrogel containing CN functionalized with an anti-inflammatory molecule to be used in situ on spinal cord injuries. The CN functionalization step was done using the drug (formononetin). The nanocomposites were characterized by morphological analysis, FTIR, Raman Spectroscopy, thermal analysis and cytotoxicity assays (MTT and HET-CAM). The nanocomposites were incorporated into gelatin methacryloyl hydrogel and exposed to UV light for photopolymerization. The volume of the formulation and the UV exposition time were also analyzed. The CN characterization showed that formononetin acted as a functionalization agent. The functionalized CN showed safe characteristics and can be incorporated in photocrosslinkable formulation. The UV exposition time for the formulation photopolymerization was compatible with the cell viability and also occurred in the injury site.
碳纳米管（CN）因其具有的电学性能和纳米级尺寸，已被用于治疗脊髓损伤。本研究旨在开发一种含有经抗炎分子功能化的碳纳米管的光聚合水凝胶，以便在脊髓损伤部位原位使用。CN 的功能化步骤使用药物（芒柄花素）进行。通过形态分析、傅里叶变换红外光谱、拉曼光谱、热分析和细胞毒性测定（MTT 和 HET-CAM）对纳米复合材料进行了表征。将纳米复合材料掺入甲基丙烯酰化明胶水凝胶中，并暴露于紫外光下进行光聚合。还分析了配方的体积和 UV 暴露时间。CN 的表征表明，芒柄花素可作为功能化试剂。功能化的 CN 表现出安全特性，并可掺入光交联配方中。配方光聚合的 UV 暴露时间与细胞活力兼容，并且也发生在损伤部位。
