Barchanska Hanna, Malejka Anna, Płonka Joanna
Department of Inorganic Chemistry, Analytical Chemistry and Electrochemistry, Silesian University of Technology, Poland.
Department of Inorganic Chemistry, Analytical Chemistry and Electrochemistry, Silesian University of Technology, Poland; Biotechnology Centre, Silesian University of Technology, Poland.
Chemosphere. 2024 Jul;359:142298. doi: 10.1016/j.chemosphere.2024.142298. Epub 2024 May 8.
Japanese radish (Raphanus sativus var. longipinnatus) plants grown under laboratory conditions were individually exposed to the same doses of atrazine (2-chloro-4-ethylamino-6-isopropylamino-1,3,5-triazine, ATR) or its main degradation products: either 2-amino-4-chloro-6-isopropylamino-1,3,5-triazine (DEA) or 2-amino-4-chloro-6-ethylamino-1,3,5-triazine (DIA) or desethyl-desisopropyl-atrazine (DEDIA) or 4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine (HA), respectively. One week after treatment in plants exposed to ATR, DIA, and DEA, their concentrations were 7.8 μg/g, 9.7 μg/g, and 14.5 μg/g, respectively, while those treated with DEDIA and HA did not contain these compounds. These results were correlated with plant amino acid profile obtained by suspect screening analysis and metabolomic "fingerprint" based on non-target analysis, obtained by liquid chromatography coupled with QTRAP triple quadrupole mass spectrometer. In all cases, both ATR and its by-products were found to interfere with the plant's amino acid profile and modify its metabolic "fingerprint". Therefore, we proved that the non-target metabolomics approach is an effective tool for investigating the hidden effects of pesticides and their transformation products, which is particularly important as these compounds may reduce the quality of edible plants.
在实验室条件下种植的日本萝卜（Raphanus sativus var. longipinnatus）植株分别暴露于相同剂量的莠去津（2-氯-4-乙氨基-6-异丙氨基-1,3,5-三嗪，ATR）或其主要降解产物：2-氨基-4-氯-6-异丙氨基-1,3,5-三嗪（DEA）、2-氨基-4-氯-6-乙氨基-1,3,5-三嗪（DIA）、去乙基-去异丙基莠去津（DEDIA）或4-（乙氨基）-2-羟基-6-（异丙氨基）-1,3,5-三嗪（HA）。在暴露于ATR、DIA和DEA的植株处理一周后，它们的浓度分别为7.8μg/g、9.7μg/g和14.5μg/g，而用DEDIA和HA处理的植株未检测到这些化合物。这些结果与通过可疑筛选分析获得的植物氨基酸谱以及基于非靶向分析的代谢组学“指纹图谱”相关，该“指纹图谱”通过液相色谱与QTRAP三重四极杆质谱仪联用获得。在所有情况下，均发现ATR及其副产物会干扰植物的氨基酸谱并改变其代谢“指纹图谱”。因此，我们证明了非靶向代谢组学方法是研究农药及其转化产物潜在影响的有效工具，这一点尤为重要，因为这些化合物可能会降低可食用植物的品质。
