Kim Wook Jin, Yang Sungyu, Choi Goya, Park Inkyu, Noh Pureum, Kim Min Jee, Moon Byeong Cheol
Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine, Daejeon 34054, Korea.
Plants (Basel). 2020 Jul 28;9(8):948. doi: 10.3390/plants9080948.
, , and are commercially valuable subtropical and tropical fruits of the Sapindaceae family. Arillus and seeds of the three species have very similar morphologies; however, the arillus of is used as the herbal medicine Longan Arillus and seeds of are used as Litchi Semen in Korean and Chinese pharmacopoeias. The adulteration of herbal medicines with inauthentic species, including the use of Aril and seed fractions acquired from a single species for two herbal medicines (e.g., Longan Arillus and Litchi Semen), is often driven by economic motives. DNA markers are a tool for the detection of adulterants in commercial products. To establish rapid and reliable assays for the genetic identification of authentic Longan Arillus and Litchi Semen, we developed DNA markers with high specificity and sensitivity based on internal transcribed spacer (ITS) sequences. The newly developed DNA markers and multiplex PCR assay may contribute to efforts to protect against adulteration, quality control, and the standardization of herbal medicines.
龙眼、荔枝和红毛丹是无患子科具有商业价值的亚热带和热带水果。这三种植物的假种皮和种子形态非常相似；然而，龙眼的假种皮被用作中药材龙眼肉，荔枝的种子在韩国和中国药典中被用作荔枝核。用不正宗的物种掺假中药材，包括将从单一物种获取的假种皮和种子部分用于两种中药材（如龙眼肉和荔枝核），往往是出于经济动机。DNA标记是检测商业产品中掺假物的一种工具。为了建立快速可靠的方法用于正品龙眼肉和荔枝核的基因鉴定，我们基于内转录间隔区（ITS）序列开发了具有高特异性和灵敏度的DNA标记。新开发的DNA标记和多重PCR检测方法可能有助于防止掺假、质量控制以及中药材标准化的工作。
