Ambrico Alfredo, Trupo Mario, Magarelli Rosaria, Balducchi Roberto, Ferraro Angelo, Hristoforou Evangelos, Marino Tiziana, Musmarra Dino, Casella Patrizia, Molino Antonio
Energy and Sustainable Economic Development Department of Sustainability, ENEA Italian National Agency for New Technologies, R.C. Trisaia S.S. 106 Jonica, 75026 Rotondella, Italy.
School of Electrical and Computer Engineering, National Technical University of Athens, Zografou Campus, 9, IroonPolytechnioustr, 15780 Athens, Greece.
Pathogens. 2020 Jul 28;9(8):613. doi: 10.3390/pathogens9080613.
Several bacteria pathogens are responsible for plant diseases causing significant economic losses. The antibacterial activity of microalgae extracts were investigated in vitro and in vivo. First, biomass composition was chemically characterized and subjected to extraction using polar/non-polar solvents. The highest extraction yield was obtained using chloroform:methanol (1:1 /) equal to 170 mg g followed by ethanol (88 mg g) and hexane (61 mg g). In vitro examination of hexane extracts of demonstrated antibacterial activity against all tested bacteria. The hexane extract showed the highest amount of β-carotene with respect to the others, so it was selected for subsequent analyses. In vivo studies were also carried out using hexane extracts of against pv. and subsp. on young tomato plants and fruits of tomato and zucchini, respectively. The treated young tomato plants exhibited a reduction of 65.7% incidence and 77.0% severity of bacterial speck spot disease. Similarly, a reduction of soft rot symptoms was observed in treated tomato and zucchini fruits with a disease incidence of 5.3% and 12.6% with respect to 90.6% and 100%, respectively, for the positive control.
几种细菌病原体可导致植物病害,造成重大经济损失。对微藻提取物的抗菌活性进行了体外和体内研究。首先,对生物量组成进行化学表征,并用极性/非极性溶剂进行提取。使用氯仿:甲醇(1:1)获得了最高提取率,为170毫克/克,其次是乙醇(88毫克/克)和己烷(61毫克/克)。对[具体微藻名称未给出]的己烷提取物进行体外检测,结果表明其对所有测试细菌均具有抗菌活性。与其他提取物相比,己烷提取物的β-胡萝卜素含量最高,因此被选用于后续分析。还分别使用[具体微藻名称未给出]的己烷提取物对番茄青枯病菌和胡萝卜软腐欧文氏菌进行了体内研究,受试植物为番茄幼苗以及番茄和西葫芦的果实。处理后的番茄幼苗细菌性斑点病发病率降低了65.7%,病情严重程度降低了77.0%。同样,在处理后的番茄和西葫芦果实中观察到软腐症状有所减轻,病害发病率分别为5.3%和12.6%,而阳性对照分别为90.6%和100%。