Tea Research Institute of Chinese Academy of Agricultural Sciences, Hangzhou, China.
Tea Research Institute, Nanjing Agricultural University, Nanjing, China.
Sci Rep. 2020 Jul 30;10(1):12858. doi: 10.1038/s41598-020-69729-x.
Colletotrichum infects diverse hosts, including tea plants, and can lead to crop failure. Numerous studies have reported that biological processes are involved in the resistance of tea plants to Colletotrichum spp. However, the molecular and biochemical responses in the host during this interaction are unclear. Cuttings of the tea cultivar Longjing 43 (LJ43) were inoculated with a conidial suspension of Colletotrichum camelliae, and water-sprayed cuttings were used as controls. In total, 10,592 differentially expressed genes (DEGs) were identified from the transcriptomic data of the tea plants and were significantly enriched in callose deposition and the biosynthesis of various phytohormones. Subsequently, 3,555 mass spectra peaks were obtained by LC-MS detection in the negative ion mode, and 27, 18 and 81 differentially expressed metabolites (DEMs) were identified in the tea leaves at 12 hpi, 24 hpi and 72 hpi, respectively. The metabolomic analysis also revealed that the levels of the precursors and intermediate products of jasmonic acid (JA) and indole-3-acetate (IAA) biosynthesis were significantly increased during the interaction, especially when the symptoms became apparent. In conclusion, we suggest that callose deposition and various phytohormone signaling systems play important roles in the tea plant-C. camelliae interaction.
炭疽菌感染多种宿主，包括茶树，并可能导致作物歉收。大量研究报告称，生物过程参与了茶树对炭疽菌的抗性。然而，在这种相互作用中，宿主的分子和生化反应尚不清楚。用炭疽菌的分生孢子悬浮液接种龙井 43 茶树插条，并以喷水插条作为对照。总共从茶树的转录组数据中鉴定出 10592 个差异表达基因（DEGs），这些基因在几丁质沉积和各种植物激素的生物合成中显著富集。随后，通过 LC-MS 在负离子模式下检测到 3555 个质谱峰，在 12 hpi、24 hpi 和 72 hpi 时分别在茶叶中鉴定出 27、18 和 81 个差异表达代谢物（DEM）。代谢组学分析还表明，茉莉酸（JA）和吲哚-3-乙酸（IAA）生物合成的前体和中间产物的水平在相互作用过程中显著增加，尤其是当症状明显时。总之，我们认为几丁质沉积和各种植物激素信号系统在茶树与炭疽菌的相互作用中发挥重要作用。
