Malavin Stas, Shmakova Lyubov, Claverie Jean-Michel, Rivkina Elizaveta
Soil Cryology Lab, Institute of Physicochemical and Biological Problems in Soil Science RAS, Pushchino, Russia Soil Cryology Lab, Institute of Physicochemical and Biological Problems in Soil Science RAS Pushchino Russia.
Aix-Marseille University, CNRS, IGS (UMR7256), IMM (FR3479), Marseille, France Aix-Marseille University, CNRS, IGS (UMR7256), IMM (FR3479) Marseille France.
Biodivers Data J. 2020 Jul 10;8:e51586. doi: 10.3897/BDJ.8.e51586. eCollection 2020.
Permafrost, frozen ground cemented with ice, occupies about a quarter of the Earth's hard surface and reaches up to 1000 metres depth. Due to constant subzero temperatures, permafrost represents a unique record of past epochs, whenever it comes to accumulated methane, oxygen isotope ratio or stored mummies of animals. Permafrost is also a unique environment where cryptobiotic stages of different microorganisms are trapped and stored alive for up to hundreds of thousands of years. Several protist strains and two giant protist viruses isolated from permafrost cores have been already described.
In this paper, we describe a collection of 35 amoeboid protist strains isolated from the samples of Holocene and Pleistocene permanently frozen sediments. These samples are stored at -18°C in the Soil Cryology Lab, Pushchino, Russia and may be used for further studies and isolation attempts. The collection strains are maintained in liquid media and may be available upon request. The paper also presents a dataset which consists of a table describing the samples and their properties (termed "Sampling events") and a table describing the isolated strains (termed "Occurrences"). The dataset is publicly available through the GBIF portal.
永久冻土是由冰固结的冻土，约占地球硬表面的四分之一，深度可达1000米。由于温度持续低于零度，无论涉及累积的甲烷、氧同位素比率还是动物木乃伊的储存，永久冻土都代表着过去时代的独特记录。永久冻土也是一个独特的环境，不同微生物的隐生阶段被捕获并存活储存长达数十万年。已经描述了从永久冻土芯中分离出的几种原生生物菌株和两种巨型原生生物病毒。
在本文中，我们描述了从全新世和更新世永久冻土沉积物样本中分离出的35种变形虫原生生物菌株的集合。这些样本保存在俄罗斯普希诺土壤低温学实验室，温度为-18°C，可用于进一步研究和分离尝试。这些集合菌株保存在液体培养基中，可根据要求提供。本文还展示了一个数据集，该数据集由一个描述样本及其特性的表格（称为“采样事件”）和一个描述分离菌株的表格（称为“出现情况”）组成。该数据集可通过GBIF门户公开获取。
