Institute of Physicochemical and Biological Problems in Soil Science RAS, Soil Cryology Lab. Russia, Institutskaya 2, 142290 Pushchino, Russia.
Eur J Protistol. 2020 Apr;73:125671. doi: 10.1016/j.ejop.2020.125671. Epub 2020 Jan 16.
Acanthamoeba castellanii species complex (genotype T4) comprises of more than ten species with unclear synonymy. Its molecular phylogeny has several conflicts with published morphological data. In this paper, we analyze morphometric traits and temperature preferences in six new strains belonging to A. castellanii complex isolated from Arctic permafrost in the framework of molecular phylogeny. This integrative approach allows us to cross-link genotypic and phenotypic variability and identify species-level boundaries inside the complex. We also analyze previously known and newly found discrepancies between the nuclear and mitochondrial gene-based phylogenies. We hypothesize that one reason for these discrepancies may be the intragenomic polymorphism of ribosomal RNA genes.
棘阿米巴 Castellanii 种复合体（基因型 T4）由十多种物种组成，其同义词尚不清楚。其分子系统发育与已发表的形态数据存在多处冲突。在本文中，我们分析了从北极永冻层分离的属于棘阿米巴 Castellanii 复合体的六个新菌株的形态特征和温度偏好，这些菌株的分离是基于分子系统发育。这种综合方法使我们能够将基因型和表型变异性联系起来，并在复合体内部确定种水平的边界。我们还分析了核基因和线粒体基因系统发育之间以前已知和新发现的差异。我们假设这些差异的一个原因可能是核糖体 RNA 基因的基因组内多态性。
