Kawasaki Takeshi, Iwasaki Tomohito, Ohya Itsuki, Hasegawa Yasuhiro, Noguchi Mitsuo, Watanabe Takafumi
Research Office Concerning the Health of Humans and Birds, Abashin 099-3119, Japan.
Department of Food Science and Human Wellness, College of Agriculture, Food and Environment Science, Rakuno Gakuen University, Ebetsu 069-8501, Japan.
J Poult Sci. 2020 Jul 25;57(3):241-245. doi: 10.2141/jpsa.0190106.
Glucose is a major circulating carbohydrate in birds and its level in the blood is often used as a biometric indicator in clinical diagnosis and various studies. Notably, hypoglycemia is often associated with Spiking Mortality Syndrome in broilers; therefore, blood glucose levels need to be correctly evaluated in clinical diagnosis. In the present study, we investigated the effect of different blood treatment methods after blood collection on chicken blood glucose measurements. The blood glucose level of plasma separated from blood cell components immediately after blood collection was used as a reference and compared with glucose levels in serum and stored plasma. The mean glucose level in plasma separated from blood cell components immediately after blood collection was 236.1±15.9 mg/dL and remained stable for at least one week in refrigerated storage (between 2°C and 5°C). However, glucose levels decreased slowly in plasma unseparated from blood cell components in storage with ice water. Mean glucose level in serum separated from blood cell components 1 h after blood collection was 206.4±9.2 mg/dL and fell to 108.3±30.0 mg/dL after 24 h. Therefore, the chicken blood serum glucose level was significantly lower than the level in plasma immediately after blood collection, regardless of elapsed time after blood collection. For the measurement of glucose in chicken blood, it is necessary to use refrigeration, use plasma from which blood cell components have been removed, and take measurements within at least 30 min.
葡萄糖是鸟类体内主要的循环碳水化合物，其血液水平常被用作临床诊断和各类研究中的生物指标。值得注意的是，低血糖常与肉鸡猝死综合征相关；因此，在临床诊断中需要正确评估血糖水平。在本研究中，我们调查了采血后不同血液处理方法对鸡血糖测量的影响。将采血后立即从血细胞成分中分离出的血浆的血糖水平用作参考，并与血清和储存血浆中的血糖水平进行比较。采血后立即从血细胞成分中分离出的血浆的平均血糖水平为236.1±15.9毫克/分升，在冷藏储存（2°C至5°C之间）至少一周内保持稳定。然而，在与血细胞成分未分离的血浆中，用冰水储存时血糖水平缓慢下降。采血1小时后从血细胞成分中分离出的血清的平均血糖水平为206.4±9.2毫克/分升，24小时后降至108.3±30.0毫克/分升。因此，无论采血后经过的时间如何，鸡血清葡萄糖水平均显著低于采血后立即测得的血浆中的水平。对于鸡血液中葡萄糖的测量，有必要使用冷藏，使用去除了血细胞成分的血浆，并在至少30分钟内进行测量。
