Alves da Paz Diego Pinha, Nagamine Márcia Kazumi, Grande Murilo Penteado Del, Leite João Vitor Pereira, Sobreira Flavia Mendonça Gonçalves, Bacchi Elfriede Marianne, Zaidan Dagli Maria Lucia
School of Veterinary Medicine and Animal Science of the University of São Paulo, Laboratory of Experimental and Comparative Oncology, Department of Pathology, Av Prof. Dr. Orlando Marques de Paiva, 87 CEP 05508-900, São Paulo, SP, Brazil.
School of Pharmaceutical Sciences of the University of São Paulo, Department of Pharmacy, Av. Professor Lineu Prestes, 580 CEP 05508-000, São Paulo, SP, Brazil.
Evid Based Complement Alternat Med. 2020 Jul 14;2020:4093206. doi: 10.1155/2020/4093206. eCollection 2020.
Lineu (Euphorbiaceae) is a tropical and subtropical ornamental and toxic plant. produces a latex that is commonly used to treat neoplasms. This study aimed to evaluate the effects of diluted latex (DETL) on human (SK-MEL-28) and canine (CBMY) melanoma cells. SK-MEL-28 (3 × 10 cells/well) and CBMY (6 × 10 cells/well) were cultivated in 96-well plates. The cells were treated with 50 l/well of dilutions (1/2, 1/4, 1/8, 1/16, 1/32, 1/64, 1/128, 1/256, and 1/512) of a standard solution containing 1 mg/mL of the latex (ETL) in DMEM. Control group cells received 50 l/well of DMEM. After 24, 48, and 72 h of treatment, cell viability was assessed by the MTT assay. There was a significant decrease in viability at 48 and 72 hours after treatment for human melanoma cells and at 24, 48, and 72 hours for canine cells, mainly in higher dilutions of ETL. Human melanoma cells presented a typical shape curve, characteristic of hormesis. To our knowledge, this is the first study showing inhibitory effects of DETL on canine melanoma cells. Therefore, DETL is a potentially new antineoplastic drug.
麻风树（大戟科）是一种热带和亚热带观赏及有毒植物。它产生的乳胶通常用于治疗肿瘤。本研究旨在评估稀释乳胶（DETL）对人（SK - MEL - 28）和犬（CBMY）黑色素瘤细胞的影响。将SK - MEL - 28（3×10细胞/孔）和CBMY（6×10细胞/孔）接种于96孔板中培养。细胞用含有1mg/mL乳胶（ETL）的标准溶液在DMEM中的稀释液（1/2、1/4、1/8、1/16、1/32、1/64、1/128、1/256和1/512）50μL/孔处理。对照组细胞接受50μL/孔的DMEM。处理24、48和72小时后，通过MTT法评估细胞活力。处理后48和72小时人黑色素瘤细胞以及24、48和72小时犬黑色素瘤细胞的活力显著下降，主要在ETL的较高稀释度时。人黑色素瘤细胞呈现出典型的剂量反应曲线，具有兴奋效应的特征。据我们所知，这是第一项显示DETL对犬黑色素瘤细胞具有抑制作用的研究。因此，DETL是一种潜在的新型抗肿瘤药物。
