Onanga Richard, Mbehang Nguema Pierre Philippe, Ndong Atome Guy Roger, Mabika Mabika Arsène, Ngoubangoye Berthelemy, Komba Tonda Wed Leslie, Obague Mbeang Jean Constant, Lebibi Jacques
Laboratory of Bacteriology, International Medical Research Center of Franceville, P.O. Box 769, Franceville, Gabon.
Laboratory of Research in Biochemistry, University of Science and Technology of Masuku, P.O. Box 913, Franceville, Gabon.
Vet Med Int. 2020 Jul 18;2020:5163493. doi: 10.1155/2020/5163493. eCollection 2020.
Antibiotic resistance occurs in the environment by multiplication and the spread of multidrug-resistant bacteria that would be due to an improper and incorrect use of antibiotics in human and veterinary medicine. The aim of this study was to establish the prevalence of producing Extended-Spectrum beta-Lactamase (ESBL) antibiotics from rats and gregarious animals in a semirural area of Gabon and to evaluate the origin of a resistance distribution in the environment from animal feces. The bacterial culture was carried out, and the identification of strains on a specific medium and the antibiotic susceptibility tests allowed establishing the prevalence. Characterization of resistance genes was performed by gene amplification after DNA extraction. On 161 feces collected in rats, 32 strains were isolated, and 11 strains of produced ESBL with a prevalence of 34.37%. Molecular tests showed that CTX-M genes 214 bp were identified in rats. The presence of CTX-M genes could have a human origin. So, the rats can carry ESBL-producing which poses a risk to human health and pets in this region of Gabon.
抗生素耐药性在环境中通过多重耐药菌的繁殖和传播而出现，这可能归因于人类和兽医学中抗生素的不当和错误使用。本研究的目的是确定加蓬一个半农村地区大鼠和群居动物中产超广谱β-内酰胺酶（ESBL）抗生素的流行情况，并评估动物粪便中环境耐药性分布的来源。进行了细菌培养，在特定培养基上对菌株进行鉴定以及抗生素敏感性试验以确定流行率。在DNA提取后通过基因扩增进行耐药基因的表征。在收集的161份大鼠粪便中，分离出32株菌株，其中11株产ESBL，流行率为34.37%。分子检测表明在大鼠中鉴定出214 bp的CTX-M基因。CTX-M基因的存在可能源于人类。因此，大鼠可携带产ESBL菌，这对加蓬该地区的人类健康和宠物构成风险。
