Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region, College of Life Sciences, Heilongjiang University, Harbin, China.
Department of Food and Environmental Engineering, East University of Heilongjiang, Harbin, China.
PLoS One. 2020 Jul 31;15(7):e0234448. doi: 10.1371/journal.pone.0234448. eCollection 2020.
Soybean root rot is a typical soil-borne disease that severely affects the yield of soybean. Funneliformis mosseae is one of the arbuscular mycorrhizal fungi(AMF) dominant strains in soybean continuous cropping soil. The aim of this study was to providing an experimental basis for the study of the molecular mechanism underlying the alleviation of the obstacles associated with the continuous cropping of soybean by AMF. In this study, F. mosseae was inoculated in soil planted with soybean infected with Fusarium oxysporum. The results showed that the incidence of soybean root rot was significantly reduced after inoculation with F. mosseae. In F. mosseae-treated samples, the significantly upregulated genes encoded transmembrane protein in fungal cell membrane. The significantly downregulated genes encoded some proteins, which took part in composition of essential component of fungal cell wall; hydrolyse cellulose and hemicellulose. The DEGs in each treatment were enriched in antigen processing and presentation, carbon fixation in photosynthetic organisms, glycolysis/gluconeogenesis, the MAPK signalling pathway, protein processing in the endoplasmic reticulum and RNA degradation. Inoculation with F. mosseae could in a variety of ways to promote the growth, development of soybean and improve disease resistance. Such as help fungal build barriers to the disease resistance of host plant and enhance their pathogenicity; damaging the structure of the pathogen; protect plant tissues and so on. This study provides an experimental basis for further research on the molecular mechanism underlying the alleviation of challenges associated with the continuous cropping of soybean by AMF.
大豆根腐病是一种典型的土传病害，严重影响大豆的产量。摩西管柄囊霉是大豆连作土壤中优势丛枝菌根真菌（AMF）之一。本研究旨在为 AMF 缓解大豆连作障碍的分子机制研究提供实验依据。本研究在接种大豆感染尖孢镰刀菌的土壤中接种摩西管柄囊霉。结果表明，接种摩西管柄囊霉后，大豆根腐病的发病率显著降低。在摩西管柄囊霉处理的样品中，真菌细胞膜跨膜蛋白编码基因的表达水平显著上调。细胞壁必需成分组成和纤维素、半纤维素水解的一些蛋白编码基因的表达水平显著下调。每个处理的差异表达基因都富集在抗原加工和呈递、光合生物中的碳固定、糖酵解/糖异生、MAPK 信号通路、内质网中的蛋白质加工和 RNA 降解途径中。摩西管柄囊霉的接种可以通过多种方式促进大豆的生长、发育和提高抗病性。例如，帮助真菌建立对宿主植物的抗病性屏障，增强其致病性；破坏病原体的结构；保护植物组织等。本研究为进一步研究 AMF 缓解大豆连作障碍的分子机制提供了实验依据。
