Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region, College of Life Sciences, Heilongjiang University, Harbin, China.
Department of Food and Environmental Engineering, East University of Heilongjiang, Harbin, China.
J Proteome Res. 2020 Sep 4;19(9):3631-3643. doi: 10.1021/acs.jproteome.0c00017. Epub 2020 Aug 17.
is easily infected with root rot in continuous cropping systems, which can severely affect crop yield. Arbuscular mycorrhizal fungi (AMF) can reduce the incidence of root rot and increase plant height and biomass indices. However, the molecular changes that occur during soybean symbiosis with AMF remain largely unknown. To better understand the molecular mechanism underlying soybean symbiosis with AMF, we performed transcriptomic and proteomic analyses to explore the changes in protein expression during a high-incidence period (79 days) in asymbiotic and symbiotic plants and to identify the key proteins that regulate the mechanism of soybean symbiosis with AMF. A total of 10 104 genes were identified in the CK-vs-F comparison, and 11 562 genes were significantly differentially expressed in the AF group compared with the F group. A total of 9488 proteins were identified, with 256 differentially expressed proteins (DEPs) in the CK-vs-F comparison and 651 DEPs in the F-vs-AF comparison. Key pathways and DEPs were found to be involved in processes associated with "phenylalanine metabolism", "plant hormone signal transduction", "plant-pathogen interaction", and "metabolic pathways". The expression of phenylalanine ammonia-lyase (PAL), calcium-dependent protein kinase (CPK), and other defense-related proteins was upregulated by , indicating that inoculation promotes the development of soybean and increases disease resistance. Our results suggest that symbiosis promotes the growth and development of soybean and increases disease resistance. This study provides new insight into the molecular basis of the mechanism by which AMF affect plant disease resistance.
大豆在连作体系中容易感染根腐病，严重影响作物产量。丛枝菌根真菌（AMF）可以降低根腐病的发病率，增加株高和生物量指数。然而，大豆与 AMF 共生过程中发生的分子变化在很大程度上尚不清楚。为了更好地理解大豆与 AMF 共生的分子机制，我们进行了转录组和蛋白质组分析，以探索非共生和共生植物在高发期（79 天）的蛋白质表达变化，并鉴定调节大豆与 AMF 共生机制的关键蛋白质。在 CK-vs-F 比较中鉴定出 10104 个基因，在 AF 组与 F 组比较中鉴定出 11562 个基因显著差异表达。共鉴定出 9488 种蛋白质，CK-vs-F 比较中有 256 个差异表达蛋白（DEPs），F-vs-AF 比较中有 651 个 DEPs。关键途径和 DEPs 被发现与“苯丙氨酸代谢”、“植物激素信号转导”、“植物-病原体相互作用”和“代谢途径”相关过程有关。接种后，苯丙氨酸解氨酶（PAL）、钙依赖性蛋白激酶（CPK）和其他防御相关蛋白的表达上调，表明接种促进了大豆的生长发育，提高了抗病性。我们的研究结果表明，共生促进了大豆的生长发育，提高了抗病性。本研究为 AMF 影响植物抗病性的机制的分子基础提供了新的见解。
