体内和体外研究甘草素对子宫内膜异位症的影响。

The effects of isoliquiritigenin on endometriosis in vivo and in vitro study.

机构信息

School of Nutrition and Health Sciences, College of Nutrition, Taipei Medical University, Taipei 11031, Taiwan.

School of Nutrition and Health Sciences, College of Nutrition, Taipei Medical University, Taipei 11031, Taiwan; Graduate Institute of Metabolism and Obesity Sciences, College of Nutrition, Taipei Medical University, Taipei 11031, Taiwan; Nutrition Research Center, Taipei Medical University Hospital, Taipei 11031, Taiwan; School of Food Safety, College of Nutrition, Taipei Medical University, Taipei 11031, Taiwan.

出版信息

Phytomedicine. 2020 Oct;77:153214. doi: 10.1016/j.phymed.2020.153214. Epub 2020 May 31.

DOI:10.1016/j.phymed.2020.153214

PMID:32736296

Abstract

BACKGROUND

Endometriosis is a common gynaecological disease characterized by growth of uterine endometrial tissue, outside the uterine cavity, on the ovaries, oviduct and pelvic peritoneum. Isoliquiritigenin (ISL) is a natural flavonoid isolated from the root of licorice (Glycyrrhiza uralensis) and shallot (Allium cepa). ISL has previously shown antioxidant, anti-inflammatory, anti-proliferation and anti-tumor activities.

PURPOSE

This study aimed to investigate the effects of ISL on endometriosis in vivo and in vitro.

METHODS

End1/E6E7 endometriosis cells were treated with ISL and β-estradiol. The MTT assay was used to detect cell viability. Cell migration was evaluated by the wound-healing assay. The expression of epithelial-to-mesenchymal transition (EMT)-related proteins were detected by western blot. Female Balb/c mice, surgically induced to have endometriosis by transplanting uterine tissue into the abdominal cavity, were treated with ISL or vehicle for 4 weeks. Lesion growth was subsequently analyzed by high-resolution ultrasound imaging. Serum and lesion inflammatory cytokines were measured by ELISA. EMT-related proteins and apoptosis-related proteins of endometriotic lesions were detected by western blot.

RESULTS

It was observed that ISL treatment inhibited the viability and migration of End1/E6E7. ISL treatment increased the expression of E-cadherin, and decreased the expression of N-cadherin, Slug and Snail. In the animal model, ISL treatment reduced the volume and weight of endometriotic lesions, decreased serum and lesion inflammatory cytokines, inhibited EMT, and induced apoptosis of the lesions.

CONCLUSION

ISL inhibited the viability, migration and EMT-related proteins of End1/E6E7 cells, reduced the volume and weight of endometriotic lesions, inhibited inflammatory cytokines and EMT, and induced apoptosis of the lesions to improve endometriosis.

摘要

背景

子宫内膜异位症是一种常见的妇科疾病，其特征是子宫内子宫内膜组织在子宫腔外、卵巢、输卵管和盆腔腹膜上生长。甘草素（ISL）是一种从甘草（Glycyrrhiza uralensis）和洋葱（Allium cepa）根部分离得到的天然类黄酮。ISL 先前显示出抗氧化、抗炎、抗增殖和抗肿瘤活性。

目的

本研究旨在探讨 ISL 对体内和体外子宫内膜异位症的影响。

方法

用 ISL 和β-雌二醇处理 End1/E6E7 子宫内膜异位症细胞。MTT 法检测细胞活力。划痕愈合试验评估细胞迁移。Western blot 检测上皮间质转化（EMT）相关蛋白的表达。通过将子宫组织移植到腹腔中来诱导子宫内膜异位症的雌性 Balb/c 小鼠，用 ISL 或载体处理 4 周。随后通过高分辨率超声成像分析病变生长。通过 ELISA 测量血清和病变炎症细胞因子。Western blot 检测子宫内膜异位症病变的 EMT 相关蛋白和凋亡相关蛋白。