Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture and Rural Affairs, Beijing, People's Republic of China.
Center of Peony, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Science, Beijing, 100081, China.
Plant Cell Rep. 2020 Nov;39(11):1425-1441. doi: 10.1007/s00299-020-02573-5. Epub 2020 Jul 31.
A total of 16 PsSPL genes were identified in tree peony. PsSPLs potentially regulated flowering time, lateral bud and seed development, and the juvenile-to-adult phase transition. SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factors are important for plant growth and development. Here, we report the identification of 16 full-length PsSPLs in tree peony (Peaonia suffruticosa Andr.) and 9 PsSPLs that have miR156 target sites. Phylogenetic analysis of the relationship of SPLs in P. suffruticosa and Arabidopsis suggested that they can be classified into six groups, and PsSPLs were highly correlated with Arabidopsis SPLs counterparts in the same group. Cis-element of promoter region analysis suggested that PsSPL genes play roles in physiological processes and developmental events. Expression analysis indicated that most PsSPL genes exhibited high expression levels in the tissues and organs examined here. The increasing expression levels of PsSPL1, PsSPL2, PsSPL8, PsSPL9, PsSPL12, and PsSPL16, and decreasing expression levels of PsSPL1A and PsSPL1B in buds over time suggested that they were probably regulated by the juvenile-to-adult phase transition. In addition, the expression profiles of PsSPL genes in different developmental buds and seeds suggested that PsSPL2, PsSPL3, PsSPL9, PsSPL10, PsSPL13, and PsSPL13A were important genes for regulating the flowering time of the tree peony; PsSPL2 and PsSPL8 might play a role in suppressing lateral bud development, and PsSPL2, PsSPL13, and PsSPL14 positively controlled grain size and number, and pod branching. These results provide a foundation for future functional analysis of PsSPL genes in tree peony growth and development.
总共在牡丹中鉴定出 16 个 PsSPL 基因。PsSPLs 可能调节开花时间、侧芽和种子发育以及幼年期到成年期的转变。SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) 转录因子对于植物的生长和发育非常重要。在这里，我们报告了在牡丹（牡丹）中鉴定出 16 个全长 PsSPL 的情况，其中 9 个 PsSPL 具有 miR156 的靶标位点。牡丹和拟南芥 SPL 之间的系统发育分析表明，它们可以分为六个组，并且牡丹 SPL 与同一组的拟南芥 SPL 对应物高度相关。启动子区域顺式元件分析表明，PsSPL 基因在生理过程和发育事件中发挥作用。表达分析表明，大多数 PsSPL 基因在检查的组织和器官中表现出高表达水平。随着时间的推移，芽中 PsSPL1、PsSPL2、PsSPL8、PsSPL9、PsSPL12 和 PsSPL16 的表达水平增加，而 PsSPL1A 和 PsSPL1B 的表达水平降低，这表明它们可能受到幼年期到成年期的转变的调节。此外，不同发育芽和种子中 PsSPL 基因的表达谱表明，PsSPL2、PsSPL3、PsSPL9、PsSPL10、PsSPL13 和 PsSPL13A 是调节牡丹开花时间的重要基因；PsSPL2 和 PsSPL8 可能在抑制侧芽发育中发挥作用，而 PsSPL2、PsSPL13 和 PsSPL14 则积极控制籽粒大小和数量以及荚分支。这些结果为牡丹生长发育中 PsSPL 基因的功能分析提供了基础。
