State Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing 400715, China.
Int J Mol Sci. 2022 Jan 20;23(3):1141. doi: 10.3390/ijms23031141.
() genes, as unique plant transcription factors, play important roles in plant developmental regulation and stress response adaptation. Although mulberry is a commercially valuable tree species, there have been few systematic studies on genes. In this work, we identified 15 full-length genes in the mulberry genome, which were distributed on 4 chromosomes. Phylogenetic analysis clustered the genes from five plants (, , , , and ) into five groups. Two zinc fingers (Zn1 and Zn2) were found in the conserved SBP domain in all of the s. Comparative analyses of gene structures and conserved motifs revealed the conservation of within a group, whereas there were significant structure differences among groups. Gene quantitative analysis showed that the expression of had tissue specificity, and had much higher expression levels in older mulberry leaves. Furthermore, transcriptome data showed that the expression levels of and were significantly increased under silkworm herbivory. Molecular experiments revealed that responded to herbivory treatment through promoting the transcription of and further upregulating the expression levels of catechin synthesis genes (, , and ).
（）基因作为独特的植物转录因子，在植物发育调控和应激适应中发挥重要作用。尽管桑树是一种具有商业价值的树种，但对 基因的系统研究较少。本研究在桑树基因组中鉴定了 15 个全长 基因，它们分布在 4 条染色体上。系统发育分析将来自 5 种植物（、、、、和）的 基因聚类为 5 个组。所有的 中都在保守的 SBP 结构域发现了两个锌指（Zn1 和 Zn2）。对基因结构和保守基序的比较分析表明，同一组内的 具有保守性，而不同组之间的结构差异显著。基因定量分析表明， 的表达具有组织特异性，且在较老的桑树叶片中具有更高的表达水平。此外，转录组数据显示，在受到家蚕取食胁迫时， 和 的表达水平显著增加。分子实验表明， 通过促进 的转录，进一步上调儿茶素合成基因（、、和）的表达水平，对取食胁迫做出响应。
