Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China (mainland).
Department of Obstetrics and Gynecology, Wuxi Maternal and Child Health Hospital Affiliated to Nanjing Medical University, Wuxi, Jiangsu, China (mainland).
Med Sci Monit. 2020 Aug 2;26:e924882. doi: 10.12659/MSM.924882.
BACKGROUND The aim of this study was to identify the differentially expressed proteins of obese patients compared with normal participants and to provide a potential target for future investigation of obesity. MATERIAL AND METHODS We enrolled 10 obese male adults and 10 matched normal subjects. Serum samples were collected to get total protein extraction, denaturation, deoxidation, and enzymatic hydrolysis. Differentially expressed proteins were distinguished with mass spectrometry after samples were labeled with iTRAQ. RESULTS A total of 9622 differentially expressed peptides were identified, corresponding to 733 proteins; 118 proteins of these showed significant differential expression, with 15 upregulated and 103 downregulated. CONCLUSIONS iTRAQ is an effective technique to identify differentially expressed proteins in obese patients. The development of obesity is correlated with a series of complex elements and mutual effects. The proteins identified in this study may provide novel directions and targets for future pathological studies of obesity.
本研究旨在鉴定肥胖患者与正常参与者之间差异表达的蛋白质，为未来肥胖症的研究提供潜在靶点。
我们招募了 10 名肥胖男性成年人和 10 名匹配的正常受试者。收集血清样本进行总蛋白提取、变性、还原和酶解。样品用 iTRAQ 标记后，通过质谱法区分差异表达蛋白。
共鉴定出 9622 个差异表达肽，对应 733 种蛋白质；其中 118 种蛋白质表达差异显著，上调 15 种，下调 103 种。
iTRAQ 是鉴定肥胖患者差异表达蛋白的有效技术。肥胖的发展与一系列复杂的因素和相互作用有关。本研究中鉴定的蛋白质可能为肥胖症的未来病理研究提供新的方向和靶点。
