Department of Chemistry, Faculty of Science, Yazd University, Yazd, Iran.
Medical Nanotechnology & Tissue Engineering Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, PO Box: 89195-999, Yazd, Iran.
Mikrochim Acta. 2020 Aug 2;187(8):479. doi: 10.1007/s00604-020-04447-9.
A novel electrochemical nanobiosensor for the detection of miR-155 (as breast cancer biomarker) is introduced . FeONPs@Ag core-shell nanoparticles were synthesized and their shape and characteristics were confirmed by scanning electron microscope (SEM) imaging, Fourier-transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD) methods. Synthesized nanoparticles were applied onto the magnetic bar carbon paste electrode and then the amine-modified anti-miR-155 (single-stranded probes) was applied on the modified electrode surface and upon hybridization with target miR-155, resveratrol (RSV) was eventually applied as an electrochemical label on the double-strand oligonucleotide. Differential pulse voltammetry (DPV) of the oxidation peak of RSV was assumed as the final signal by sweeping potential from 0 to 0.6 V (vs. Ag/AgCl). The fabrication process was optimized through a series of experiments and the optimized process was confirmed using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The linear range of the fabricated nanobiosensor was 0.5 fM to 1.0 nM and the detection limit was 0.15 fM. The nanobiosensor was able to pass reproducibility and specificity tests using different types of mismatched target sequences.Spiked real samples of human serum were used to confirm that the nanobiosensor enables detection of miR-155 without any significant interferences from other moieties and molecules. Finally, the molecular dynamics simulation of the RSV interaction with single- and double-stranded oligonucleotide was performed and confirmed the preferential binding of RSV to double-stranded DNA; therefore, it can be used as the electrochemical label of DNA and/or miRNA hybridization-based biosensors. Graphical abstract.
一种用于检测 miR-155（作为乳腺癌生物标志物）的新型电化学纳米生物传感器被介绍。FeONPs@Ag 核壳纳米粒子被合成，通过扫描电子显微镜（SEM）成像、傅里叶变换红外光谱（FTIR）和 X 射线衍射（XRD）方法确认其形状和特性。合成的纳米粒子被应用于磁性棒碳糊电极上，然后将胺修饰的抗 miR-155（单链探针）应用于修饰电极表面，在与靶 miR-155 杂交后，白藜芦醇（RSV）最终作为电化学标签应用于双链寡核苷酸上。通过从 0 到 0.6 V（相对于 Ag/AgCl）的电势扫描，将 RSV 的氧化峰的差分脉冲伏安法（DPV）作为最终信号。通过一系列实验优化了制备过程，并使用循环伏安法（CV）和电化学阻抗谱（EIS）对优化过程进行了验证。所制备的纳米生物传感器的线性范围为 0.5 fM 至 1.0 nM，检测限为 0.15 fM。该纳米生物传感器能够通过使用不同类型的错配靶序列进行重复性和特异性测试。使用人血清的实际加标样品证实，纳米生物传感器能够在没有其他物质和分子的显著干扰的情况下检测 miR-155。最后，对 RSV 与单链和双链寡核苷酸相互作用的分子动力学模拟进行了，并证实 RSV 优先与双链 DNA 结合；因此，它可以用作 DNA 和/或 miRNA 杂交为基础的生物传感器的电化学标签。
