Parfenov Vladislav A, Khesuani Yusef D, Petrov Stanislav V, Karalkin Pavel A, Koudan Elizaveta V, Nezhurina Elizaveta K, Pereira Frederico DAS, Krokhmal Alisa A, Gryadunova Anna A, Bulanova Elena A, Vakhrushev Igor V, Babichenko Igor I, Kasyanov Vladimir, Petrov Oleg F, Vasiliev Mikhail M, Brakke Kenn, Belousov Sergei I, Grigoriev Timofei E, Osidak Egor O, Rossiyskaya Ekaterina I, Buravkova Ludmila B, Kononenko Oleg D, Demirci Utkan, Mironov Vladimir A
Laboratory for Biotechnological Research "3D Bioprinting Solutions", Moscow, Russia.
A.A. Baikov Institute of Metallurgy and Material Science, Russian Academy of Sciences, Moscow, Russia.
Sci Adv. 2020 Jul 15;6(29):eaba4174. doi: 10.1126/sciadv.aba4174. eCollection 2020 Jul.
Magnetic levitational bioassembly of three-dimensional (3D) tissue constructs represents a rapidly emerging scaffold- and label-free approach and alternative conceptual advance in tissue engineering. The magnetic bioassembler has been designed, developed, and certified for life space research. To the best of our knowledge, 3D tissue constructs have been biofabricated for the first time in space under microgravity from tissue spheroids consisting of human chondrocytes. Bioassembly and sequential tissue spheroid fusion presented a good agreement with developed predictive mathematical models and computer simulations. Tissue constructs demonstrated good viability and advanced stages of tissue spheroid fusion process. Thus, our data strongly suggest that scaffold-free formative biofabrication using magnetic fields is a feasible alternative to traditional scaffold-based approaches, hinting a new perspective avenue of research that could significantly advance tissue engineering. Magnetic levitational bioassembly in space can also advance space life science and space regenerative medicine.
三维（3D）组织构建体的磁悬浮生物组装代表了一种迅速兴起的无支架、无标记方法，是组织工程领域的一项概念性进展。磁生物组装器已被设计、开发并通过了太空生命研究认证。据我们所知，首次在太空微重力环境下，利用由人类软骨细胞组成的组织球状体生物制造出了3D组织构建体。生物组装和组织球状体的顺序融合与已开发的预测数学模型和计算机模拟结果高度吻合。组织构建体显示出良好的活力以及组织球状体融合过程的高级阶段。因此，我们的数据有力地表明，利用磁场进行无支架成型生物制造是传统基于支架方法的可行替代方案，为可能显著推进组织工程的新研究方向提供了思路。太空磁悬浮生物组装也能够推动太空生命科学和太空再生医学的发展。
