İyigün Sezgi, Görgülü Nimet Gül, Doğan Başak
Department of Periodontology, Faculty of Dentistry, Marmara University, Maltepe, Istanbul, 34854, Türkiye.
BMC Oral Health. 2025 Feb 19;25(1):262. doi: 10.1186/s12903-025-05610-5.
Matrix metalloproteinase-9 (MMP-9), total superoxide dismutase (T-SOD) and sirtuin (SIRT)-1 are interrelated molecules linked with oxidative stress and periodontitis pathogenesis which may have implications for both the diagnosis of periodontitis or efficacy of periodontal treatment. This study aimed to evaluate salivary and serum MMP-9, T-SOD, and SIRT-1 levels in stage 3 periodontitis patients (P-S3) following non-surgical periodontal treatment (NSPT).
Forty-nine subjects, comprising 17 healthy, 16 P-S3 Grade B (P-S3GB), and 16 P-S3 Grade C (P-S3GC) subjects, participated in the study. Clinical periodontal parameters, salivary and serum MMP-9, T-SOD and SIRT-1 levels were evaluated at baseline from all participants and at 3-months from periodontitis patients. Enzyme-linked immunosorbent assay (ELISA) was used to assess the levels of the biochemical parameters.
All clinical periodontal parameters improved in both periodontitis groups (p < 0.05) after NSPT. In P-S3GB and GC groups, salivary levels of MMP-9 and T-SOD were higher than healthy controls (p < 0.05) and decreased following NSPT (p < 0.05). P-S3GC group had higher serum MMP-9 levels than the healthy ones (p < 0.05). Salivary SIRT-1 and serum T-SOD levels were similar among groups (p > 0.05). After NSPT, only in P-S3GB group, salivary SIRT-1 decreased, and serum T-SOD increased (p < 0.05). Salivary T-SOD and MMP-9 showed positive correlations with clinical parameters (p < 0.05).
The study results suggest that MMP-9 and T-SOD are potential biomarkers for diagnosing P-S3GB and GC and evaluating the efficacy of NSPT. However, further research is necessary to validate these findings across all grades and stages of periodontitis.
This study was retrospectively registered on 13/02/2024 at Clinical trials.gov with the number NCT06255470.
基质金属蛋白酶-9（MMP-9）、总超氧化物歧化酶（T-SOD）和沉默调节蛋白（SIRT）-1是与氧化应激和牙周炎发病机制相关的相互关联分子，这可能对牙周炎的诊断或牙周治疗的疗效都有影响。本研究旨在评估非手术牙周治疗（NSPT）后3期牙周炎患者（P-S3）的唾液和血清MMP-9、T-SOD和SIRT-1水平。
49名受试者参与了本研究，包括17名健康受试者、16名P-S3 B级（P-S3GB）受试者和16名P-S3 C级（P-S3GC）受试者。在基线时对所有参与者进行临床牙周参数、唾液和血清MMP-9、T-SOD和SIRT-1水平评估，并在牙周炎患者治疗3个月后再次评估。采用酶联免疫吸附测定（ELISA）法评估生化参数水平。
NSPT后，两个牙周炎组的所有临床牙周参数均有所改善（p < 0.05）。在P-S3GB组和P-S3GC组中，唾液MMP-9和T-SOD水平高于健康对照组（p < 0.05），NSPT后降低（p < 0.05）。P-S3GC组血清MMP-9水平高于健康组（p < 0.05）。各组间唾液SIRT-1和血清T-SOD水平相似（p > 0.05）。NSPT后，仅在P-S3GB组中，唾液SIRT-1降低，血清T-SOD升高（p < 0.05）。唾液T-SOD和MMP-9与临床参数呈正相关（p < 0.05）。
研究结果表明，MMP-9和T-SOD是诊断P-S3GB和P-S3GC以及评估NSPT疗效的潜在生物标志物。然而，需要进一步研究以在牙周炎的所有分级和阶段验证这些发现。
本研究于2024年2月13日在ClinicalTrials.gov上进行回顾性注册，注册号为NCT06255470。
