Poole Stephanie Francoi, Pitondo-Silva André, Oliveira-Silva Mariana, Moris Izabela C M, Gomes Erica Alves
School of Dentistry, University of Ribeirão Preto (UNAERP), Ribeirão Preto, SP, Brazil.
Postgraduate Program in Environmental Technology, University of Ribeirão Preto, Ribeirão Preto, SP, Brazil.
J Mech Behav Biomed Mater. 2020 Nov;111:104010. doi: 10.1016/j.jmbbm.2020.104010. Epub 2020 Jul 25.
Ceramics are used in oral rehabilitation; however, these materials are prone to formation of biofilms that may cause periodontal diseases. This study aimed to evaluate the influence of distinct surface treatments on ceramic surface roughness and biofilm formation of oral bacteria (Prevotella intermedia). Eighty-four specimens of the following four ceramic systems were produced: LC - leucite-based glass ceramic, LD - lithium disilicate-based glass ceramic, LSZ - glass ceramic based on zirconia-reinforced lithium silicate, and ZR - monolithic zirconia. These were submitted to three different surface treatment protocols: C - control, G - glazing, and GDB - grinding with diamond bur (n = 7). The surface characteristics were assessed using a confocal laser microscope (Ra) and a scanning electron microscope (SEM). Thereafter, the groups were contaminated with a bacterial strain of P. intermedia ATCC 25611. The biofilms formed were quantified by counting the colony forming units (CFUs) and analyzed with scanning electron microscope (SEM) and confocal laser scanning microscope (CLSM). Data were analyzed by using a 2-way ANOVA and the Tukey test (ɑ = 0.05). Results showed that greater roughness was associated with GDB (p < 0.05). The same was also true for the ceramic material ZR (p < 0.05). There was a statistical significant difference in the CFU counts between the materials (p < 0.05) that revealed a greater amount of bacterial adhesion in the LC and ZR groups (p > 0.05). Thus, it was suggested that the surface roughness of the ceramic materials favored bacterial adhesion; and thus, finishing of ceramic surfaces with GDB should be avoided.
陶瓷被用于口腔修复；然而，这些材料容易形成生物膜，可能导致牙周疾病。本研究旨在评估不同表面处理对陶瓷表面粗糙度以及口腔细菌（中间普氏菌）生物膜形成的影响。制备了以下四种陶瓷系统的84个样本：LC - 白榴石基微晶玻璃、LD - 二硅酸锂基微晶玻璃、LSZ - 氧化锆增强硅酸锂基微晶玻璃和ZR - 整体式氧化锆。将这些样本分别进行三种不同的表面处理方案：C - 对照、G - 上釉和GDB - 用金刚石车针打磨（n = 7）。使用共聚焦激光显微镜（Ra）和扫描电子显微镜（SEM）评估表面特性。此后，用中间普氏菌ATCC 25611的细菌菌株污染各实验组。通过计数菌落形成单位（CFU）对形成的生物膜进行定量，并使用扫描电子显微镜（SEM）和共聚焦激光扫描显微镜（CLSM）进行分析。数据采用双向方差分析和Tukey检验进行分析（ɑ = 0.05）。结果表明，GDB处理后的粗糙度更大（p < 0.05）。陶瓷材料ZR也是如此（p < 0.05）。材料之间的CFU计数存在统计学显著差异（p < 0.05），这表明LC组和ZR组的细菌黏附量更大（p > 0.05）。因此，提示陶瓷材料的表面粗糙度有利于细菌黏附；因此，应避免使用GDB对陶瓷表面进行精加工。
