咖啡酸苯乙酯（CAPE）通过调节 BCL2/BAX 基因诱导浆液性卵巢癌细胞 OV7 细胞凋亡。
Caffeic Acid Phenethyl Ester (CAPE) Induced Apoptosis in Serous Ovarian Cancer OV7 Cells by Deregulation of BCL2/BAX Genes.
机构信息
Department of Pathology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia in Katowice, Ostrogórska 30, 41-200 Sosnowiec, Poland.
Faculty of Biomedical Engineering, Department of Biosensors and Processing of Biomedical Signals, Silesian University of Technology, Roosevelta 40, 41-800 Zabrze, Poland.
出版信息
Molecules. 2020 Jul 31;25(15):3514. doi: 10.3390/molecules25153514.
Ovarian cancer has the worst prognosis among all gynecological cancers. Therefore, it seems reasonable to seek new drugs that may improve the effectiveness of treatment or mitigate the adverse effects of chemotherapy. Caffeic acid phenethyl ester (CAPE) has many beneficial biological properties. The aim of the study was to assess the anticancer properties of CAPE against serum ovarian carcinoma cells. The morphology of the cells was evaluated in H-E staining and in transmission electron microscopy. The cytotoxic and proapoptotic activity of CAPE was investigated by using the XTT-NR-SRB assay, qRT-PCR analysis of BAX/BCL2 expression, and by cytometric evaluation. CAPE causes constriction in OV7 cells, numerous granulomas were observed in the cytoplasm, the cell nuclei were pyknotic. Autophagosomal vacuoles could suggest the occurrence of aponecrosis. CAPE significantly decreased the lysosomal activity and the total synthesis of cellular proteins. CAPE exhibited, dose and time dependent, cytotoxic activity against OV7 serum ovarian cancer cells. In OV7 cells CAPE induced apoptosis via dysregulation of BAX/BCL2 balance, while activated proapoptotic BAX gene expression level was 10 times higher than BCL2.
卵巢癌是妇科癌症中预后最差的一种。因此，寻找可能提高治疗效果或减轻化疗副作用的新药似乎是合理的。咖啡酸苯乙酯 (CAPE) 具有许多有益的生物学特性。本研究旨在评估 CAPE 对血清卵巢癌细胞的抗癌特性。用 H-E 染色和透射电子显微镜评估细胞形态。通过 XTT-NR-SRB 测定、BAX/BCL2 表达的 qRT-PCR 分析以及细胞计评估研究 CAPE 的细胞毒性和促凋亡活性。CAPE 导致 OV7 细胞收缩，细胞质中观察到许多肉芽肿，细胞核固缩。自噬小泡可能提示发生了细胞坏死。CAPE 显著降低溶酶体活性和细胞总蛋白质合成。CAPE 对 OV7 血清卵巢癌细胞具有剂量和时间依赖性的细胞毒性作用。在 OV7 细胞中，CAPE 通过 BAX/BCL2 平衡失调诱导细胞凋亡，而激活的促凋亡 BAX 基因表达水平比 BCL2 高 10 倍。
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