羟基酪醇对脂多糖诱导的牛乳腺上皮细胞炎症和氧化应激的影响：一种治疗牛乳腺炎的天然工具。

Effects of Hydroxytyrosol against Lipopolysaccharide-Induced Inflammation and Oxidative Stress in Bovine Mammary Epithelial Cells: A Natural Therapeutic Tool for Bovine Mastitis.

作者信息

Fusco Roberta, Cordaro Marika, Siracusa Rosalba, Peritore Alessio Filippo, D'Amico Ramona, Licata Patrizia, Crupi Rosalia, Gugliandolo Enrico

机构信息

Department of Chemical, Biological, Pharmaceutical and Environmental Science, University of Messina, 98166 Messina, Italy.

Department of Veterinary Science, University of Messina, 98168 Messina, Italy.

出版信息

Antioxidants (Basel). 2020 Aug 3;9(8):693. doi: 10.3390/antiox9080693.

BACKGROUND

Bovine mastitis is a growing health problem, affecting both welfare of dairy cattle and milk production. It often leads to chronic infections, disturbing the quality of milk and resulting in cow death. Thus, it has a great economic impact for breeders.

METHODS

In this study, we evaluated the protective effect of hydroxytyrosol-a natural molecule which is the major constituent of many phyto-complexes-in an in vitro model of mastitis induced by LPS (1μg/mL).

RESULTS

Our results showed that hydroxytyrosol (10 and 25 μM) was able to prevent the oxidative stress induced by LPS (intracellular ROS, GSH and NOX-1) and the consequently inflammatory response (TNF-α, IL-1β and IL-6). The protective effect of hydroxytyrosol is also related to the enhancement of endogenous antioxidant systems (Nrf2, HO-1, NQO-1 and Txnrd1). Moreover, hydroxytyrosol showed an important protective effect on cell functionality (α-casein S1, α-casein S2 and β-casein).

CONCLUSIONS

Taken together, our results showed a significant protective effect of hydroxytyrosol on oxidative stress and inflammatory response in MAC-T cells. Thus, we indicated a possible important therapeutic role for hydroxytyrosol in the prevention or management of bovine mastitis.

背景

奶牛乳腺炎是一个日益严重的健康问题，影响奶牛的福利和牛奶产量。它常常导致慢性感染，扰乱牛奶质量并导致奶牛死亡。因此，它对养殖者有巨大的经济影响。

方法

在本研究中，我们在脂多糖（1μg/mL）诱导的乳腺炎体外模型中评估了羟基酪醇（一种天然分子，是许多植物复合物的主要成分）的保护作用。

结果

我们的结果表明，羟基酪醇（10和25μM）能够预防脂多糖诱导的氧化应激（细胞内活性氧、谷胱甘肽和NOX-1）以及随之而来的炎症反应（肿瘤坏死因子-α、白细胞介素-1β和白细胞介素-6）。羟基酪醇的保护作用还与内源性抗氧化系统（核因子E2相关因子2、血红素加氧酶-1、醌氧化还原酶-1和硫氧还蛋白还原酶1）的增强有关。此外，羟基酪醇对细胞功能（α-酪蛋白S1、α-酪蛋白S2和β-酪蛋白）显示出重要的保护作用。