Tang Jen-Yang, Wu Kuang-Han, Wang Yen-Yun, Farooqi Ammad Ahmad, Huang Hurng-Wern, Yuan Shyng-Shiou F, Jian Ru-In, Tsao Li-Yi, Chen Po-An, Chang Fang-Rong, Cheng Yuan-Bin, Hu Hao-Chun, Chang Hsueh-Wei
Department of Radiation Oncology, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
Department of Radiation Oncology, Kaohsiung Medical University Hospital, Kaohsiung 80708, Taiwan.
Antioxidants (Basel). 2020 Aug 3;9(8):694. doi: 10.3390/antiox9080694.
Some lichens provide the resources of common traditional medicines and show anticancer effects. However, the anticancer effect of () is rarely investigated, especially for oral cancer cells. The aim of this study was to investigate the cell killing function of methanol extracts of (MEUB) against oral cancer cells. MEUB shows preferential killing against a number of oral cancer cell lines (Ca9-22, OECM-1, CAL 27, HSC3, and SCC9) but rarely affects normal oral cell lines (HGF-1). Ca9-22 and OECM-1 cells display the highest sensitivity to MEUB and were chosen for concentration effect and time course experiments to address its cytotoxic mechanisms. MEUB induces apoptosis of oral cancer cells in terms of the findings from flow cytometric assays and Western blotting, such as subG1 accumulation, annexin V detection, and pancaspase activation as well as poly (ADP-ribose) polymerase (PARP) cleavage. MEUB induces oxidative stress and DNA damage of oral cancer cells following flow cytometric assays, such as reactive oxygen species (ROS)/mitochondrial superoxide (MitoSOX) production, mitochondrial membrane potential (MMP) depletion as well as overexpression of γH2AX and 8-oxo-2'deoxyguanosine (8-oxodG). All MEUB-induced changes in oral cancer cells were triggered by oxidative stress which was validated by pretreatment with antioxidant -acetylcysteine (NAC). In conclusion, MEUB causes preferential killing of oral cancer cells and is associated with oxidative stress, apoptosis, and DNA damage.
一些地衣提供了常见传统药物的资源，并显示出抗癌作用。然而，（某种地衣）的抗癌作用很少被研究，尤其是对口腔癌细胞。本研究的目的是研究（某种地衣）甲醇提取物（MEUB）对口腔癌细胞的细胞杀伤功能。MEUB对多种口腔癌细胞系（Ca9-22、OECM-1、CAL 27、HSC3和SCC9）显示出优先杀伤作用，但很少影响正常口腔细胞系（HGF-1）。Ca9-22和OECM-1细胞对MEUB表现出最高的敏感性，因此被选用于浓度效应和时间进程实验，以探讨其细胞毒性机制。根据流式细胞术分析和蛋白质免疫印迹的结果，MEUB诱导口腔癌细胞凋亡，如亚G1期积累、膜联蛋白V检测、泛半胱天冬酶激活以及聚（ADP-核糖）聚合酶（PARP）裂解。根据流式细胞术分析，MEUB诱导口腔癌细胞氧化应激和DNA损伤，如活性氧（ROS）/线粒体超氧化物（MitoSOX）产生、线粒体膜电位（MMP）耗竭以及γH2AX和8-氧代-2'-脱氧鸟苷（8-oxodG）的过表达。MEUB诱导的口腔癌细胞的所有变化均由氧化应激触发，这通过用抗氧化剂N-乙酰半胱氨酸（NAC）预处理得到验证。总之，MEUB导致口腔癌细胞的优先杀伤，并与氧化应激、细胞凋亡和DNA损伤有关。
