Falgout B, Ketner G
Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218.
J Virol. 1988 Feb;62(2):622-5. doi: 10.1128/JVI.62.2.622-625.1988.
H2dl802, H2dl807, and H5dl1021 are defective deletion mutants of human adenovirus which do not make the capsid protein fiber yet which can make substantial amounts of virus particles. Virions made by the mutants contain very little fiber (which comes from helper virus contaminants in the deletion virus stocks): less than 6% as much as that contained by wild-type virions. This demonstrates that fiber is not an essential structural component of the adenovirus virion and suggests that fiber is nonessential for virion assembly. These fiber-deficient particles are poorly adsorbed to cells, consistent with the proposed role of fiber in virus attachment. Further, virion protein precursors, including that of the virion protease, are poorly processed in these particles, suggesting a relationship between the presence of fiber and the maturation of the virus particle.
H2dl802、H2dl807和H5dl1021是人类腺病毒的缺陷性缺失突变体，它们不产生衣壳蛋白纤维，但能产生大量病毒颗粒。这些突变体产生的病毒粒子含有极少的纤维（来自缺失病毒储备中的辅助病毒污染物）：不到野生型病毒粒子所含纤维量的6%。这表明纤维不是腺病毒病毒粒子的必需结构成分，并提示纤维对于病毒粒子组装并非必需。这些缺乏纤维的颗粒与细胞的吸附能力很差，这与纤维在病毒附着中所起的作用相符。此外，病毒粒子蛋白前体，包括病毒粒子蛋白酶的前体，在这些颗粒中的加工处理很差，这表明纤维的存在与病毒粒子的成熟之间存在关联。
