Yeh-Kai L, Akusjärvi G, Aleström P, Pettersson U, Tremblay M, Weber J
J Mol Biol. 1983 Jun 15;167(1):217-22. doi: 10.1016/s0022-2836(83)80044-8.
The temperature-sensitive adenovirus type 2 mutant H2ts1 is defective for polypeptide processing at the non-permissive temperature. We have in the present study mapped the mutation by marker rescue and DNA sequencing techniques: the mutation is a C/T transition located at map co-ordinate 61.1. Previous sequencing studies have identified an uninterrupted translational reading frame in this part of the adenovirus genome, encoding a hypothetical 23 X 10(3) Mr polypeptide. The mutation leads to a proline/leucine substitution in the 23 X 10(3) Mr polypeptide.
温度敏感型腺病毒2型突变体H2ts1在非允许温度下多肽加工存在缺陷。在本研究中，我们通过标记拯救和DNA测序技术对该突变进行了定位：该突变是位于图谱坐标61.1处的C/T转换。先前的测序研究已在腺病毒基因组的这一部分鉴定出一个不间断的翻译阅读框，编码一种假定的23×10³Mr多肽。该突变导致23×10³Mr多肽中脯氨酸/亮氨酸的替换。
