Koçoğlu İrem Okman, Erden Pınar Esra, Kılıç Esma
Department of Chemistry, Faculty of Science, Ankara University, 06100, Ankara, Turkey.
Anal Methods. 2020 Aug 14;12(30):3802-3812. doi: 10.1039/d0ay00802h. Epub 2020 Jul 15.
This study presents the development of disposable biosensors employed in the determination of histamine in fish samples. Screen printed carbon electrodes (SPCEs) were first modified with a mixture of titanium dioxide nanoparticles (TiO), carboxylated multiwalled carbon nanotubes (c-MWCNTs), hexaammineruthenium(iii) chloride (RU) and chitosan (CS). Diamine oxidase (DAO) or monoamine oxidase (MAO) enzymes were further immobilized onto the TiO-c-MWCNT-RU-CS/SPCEs via 1-ethyl-3-(dimethylaminopropyl) carbodiimide hydrochloride (EDC) and hydroxysuccinimide (NHS) chemistry for the fabrication of the biosensors. The morphological and electrochemical properties of the proposed biosensors were studied using scanning electron microscopy (SEM), energy dispersive X-ray (EDX) spectroscopy, cyclic voltammetry (CV), chronoamperometry and electrochemical impedance spectroscopy (EIS). A performance comparison of two biosensors indicated that the one based on DAO had a linear concentration range from 9.9 × 10 to 1.1 × 10 M and the other based on MAO, from 5.6 × 10 to 1.1 × 10 M for histamine. The sensitivity of the DAO based biosensor was almost 1.5 times higher than that of the MAO based biosensor. The proposed biosensors were successfully employed to determine histamine in fish samples and the recoveries were between 100.0% and 104.6%.
本研究介绍了用于测定鱼样中组胺的一次性生物传感器的研制。首先用二氧化钛纳米颗粒（TiO）、羧基化多壁碳纳米管（c-MWCNTs）、六氨合钌（III）氯化物（RU）和壳聚糖（CS）的混合物对丝网印刷碳电极（SPCEs）进行修饰。通过1-乙基-3-（二甲基氨基丙基）碳二亚胺盐酸盐（EDC）和N-羟基琥珀酰亚胺（NHS）化学方法将二胺氧化酶（DAO）或单胺氧化酶（MAO）进一步固定在TiO-c-MWCNT-RU-CS/SPCEs上，以制备生物传感器。使用扫描电子显微镜（SEM）、能量色散X射线（EDX）光谱、循环伏安法（CV）、计时电流法和电化学阻抗谱（EIS）研究了所提出的生物传感器的形态和电化学性质。两种生物传感器的性能比较表明，基于DAO的生物传感器对组胺的线性浓度范围为9.9×10至1.1×10 M，基于MAO的生物传感器对组胺的线性浓度范围为5.6×10至1.1×10 M。基于DAO的生物传感器的灵敏度几乎是基于MAO的生物传感器的1.5倍。所提出的生物传感器成功用于测定鱼样中的组胺，回收率在100.0%至104.6%之间。
