通过免疫过氧化物酶法在组织切片中对白吉利毛孢子菌进行免疫组织化学鉴定。

Immunohistochemical identification of Trichosporon beigelii in histologic section by immunoperoxidase method.

作者信息

Kobayashi M, Kotani S, Fujishita M, Taguchi H, Moriki T, Enzan H, Miyoshi I

机构信息

Department of Medicine, Kochi Medical School, Japan.

出版信息

Am J Clin Pathol. 1988 Jan;89(1):100-5. doi: 10.1093/ajcp/89.1.100.

DOI:10.1093/ajcp/89.1.100

PMID:3276138

Abstract

An indirect immunoperoxidase method capable of identifying an opportunistic fungus, Trichosporon beigelii, in formalin-fixed, paraffin-embedded tissue has been developed. The authors studied autopsy materials from 19 patients with various fungal infections, including 3 patients with disseminated T. beigelii infection, 8 patients with localized or systemic candidiasis, 4 patients with invasive aspergillosis, 2 patients with pulmonary mucormycosis, and 2 patients with systemic cryptococcosis. T. beigelii could be successfully visualized in tissue sections from all three patients with this infection and a patient with systemic candidiasis who was found to have concomitant infection by T. beigelii. The capsule of Cryptococcus neoformans was also stained, indicating the presence of cross-reactive antigen between T. beigelii and C. neoformans. C. neoformans-absorbed antiserum was reactive with T. beigelii but not with other fungi, including C. neoformans.

摘要

已开发出一种间接免疫过氧化物酶方法，可在福尔马林固定、石蜡包埋的组织中鉴定机会性真菌白吉利丝孢酵母。作者研究了19例各种真菌感染患者的尸检材料，其中包括3例播散性白吉利丝孢酵母感染患者、8例局限性或全身性念珠菌病患者、4例侵袭性曲霉病患者、2例肺毛霉病患者和2例系统性隐球菌病患者。在所有3例该感染患者以及1例被发现同时感染白吉利丝孢酵母的全身性念珠菌病患者的组织切片中，均可成功观察到白吉利丝孢酵母。新型隐球菌的荚膜也被染色，表明白吉利丝孢酵母与新型隐球菌之间存在交叉反应性抗原。新型隐球菌吸收的抗血清与白吉利丝孢酵母有反应，但与包括新型隐球菌在内的其他真菌无反应。