Fukuzawa M, Inaba H, Hayama M, Sakaguchi N, Sano K, Ito M, Hotchi M
Department of Pathology, Shinshu University School of Medicine, Nagano, Japan.
Virchows Arch. 1995;427(4):407-14. doi: 10.1007/BF00199390.
This study was performed to identify pathological fungi of eight species [Aspergillus fumigatus, Candida albicans, Torulopsis (Candida) glabrata, Cryptococcus neoformans, Fusarium anthophilum, Rhizopus oryzae, Sporothrix schenckii and Trichosporon beigelii] in formalin-fixed, paraffin-embedded tissue sections by indirect immunoperoxidase staining. Mature albino rabbits were immunized with formalin-killed organisms. Antibodies were prepared by precipitation. Immunoperoxidase staining was applied to the paraffin-embedded tissue sections of experimentally infected mice and human autopsy and surgical specimens. Although the cell walls of each fungus stained clearly, many cross-reactivities appeared. However, it was possible to obtain specificity for the eight species by absorption and dilution of the antisera.
本研究旨在通过间接免疫过氧化物酶染色法，在福尔马林固定、石蜡包埋的组织切片中鉴定8种致病真菌[烟曲霉、白色念珠菌、光滑念珠菌（原假丝酵母菌）、新型隐球菌、嗜花镰刀菌、米根霉、申克孢子丝菌和白吉利丝孢酵母]。用福尔马林灭活的菌体免疫成年白化兔。通过沉淀法制备抗体。将免疫过氧化物酶染色应用于实验感染小鼠的石蜡包埋组织切片以及人体尸检和手术标本。虽然每种真菌的细胞壁均染色清晰，但出现了许多交叉反应。不过，通过抗血清的吸收和稀释，有可能获得针对这8种真菌的特异性。
