Olamazadeh Mohammad Hassan, Esfarjani Fahimeh, Marandi Sayed Mohammad, Zamani Saeed, Rarani Fahimeh Zamani, Sharifi Mohammadreza
Department of Exercise Physiology, Faculty of Sport Science, University of Isfahan, Isfahan, Iran.
Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Int J Prev Med. 2025 Feb 26;16:13. doi: 10.4103/ijpvm.ijpvm_79_24. eCollection 2025.
Angiogenesis is crucial in the recovery and maintenance of heart function. Irisin may mediate the cardiac health-promoting impact of exercise training. The aim of this study was to comparatively assess VEGF gene/protein expression in the heart tissue and correlations with serum irisin levels following resistance training, in comparison to exogenous irisin injection.
Twenty-one mice were randomly assigned to the three group ( = 7 for each group): control, resistance exercise, and irisin. Exercised mice, for 8 weeks, three sessions per week and four sets of five repeats for each session were considered and mice climbed up a 1-m-height ladder with a slope of 80 degrees with a weight equal to 30% of mouse's body weight fastened to their tails and gradually increased up twofold of body weight. The Irisin group received 100 μg/kg/week irisin for 8 weeks, intraperitoneally. The cardiac expression of the gene, by real-time PCR, the level of VEGF protein, by IHC (immunohistochemistry) and western blot analysis, and serum irisin concentration, by ELISA, were evaluated.
The expression of the VEGF gene and protein, as well as serum Irisin levels, increased in all experimental mice compared to the control group ( < 0.05). Pearson's correlation coefficient data indicated a positive correlation between the analyzed parameters in each group ( < 0.05 and > 0).
There appears to be an interaction between resistance exercise and cardiac angiogenesis factors, mediated by irisin. So, irisin could be considered in cardiovascular health interventions, aiming to target specific molecules or pathways.
血管生成在心脏功能的恢复和维持中至关重要。鸢尾素可能介导运动训练对心脏健康的促进作用。本研究的目的是比较评估抗阻训练后心脏组织中血管内皮生长因子（VEGF）基因/蛋白表达及其与血清鸢尾素水平的相关性，并与外源性鸢尾素注射进行比较。
将21只小鼠随机分为三组（每组n = 7）：对照组、抗阻运动组和鸢尾素组。对运动组小鼠进行为期8周的训练，每周训练3次，每次训练4组，每组重复5次，小鼠尾巴系上相当于其体重30%的重物，爬上坡度为80度、高1米的梯子，随后逐渐将重物重量增加至体重的两倍。鸢尾素组小鼠腹腔注射100 μg/kg/周的鸢尾素，持续8周。通过实时聚合酶链反应（PCR）评估VEGF基因的心脏表达，通过免疫组织化学（IHC）和蛋白质印迹分析评估VEGF蛋白水平，通过酶联免疫吸附测定（ELISA）评估血清鸢尾素浓度。
与对照组相比，所有实验小鼠的VEGF基因和蛋白表达以及血清鸢尾素水平均升高（P < 0.05）。Pearson相关系数数据表明，每组分析参数之间呈正相关（P < 0.05且r > 0）。
抗阻运动与心脏血管生成因子之间似乎存在由鸢尾素介导的相互作用。因此，在旨在靶向特定分子或途径的心血管健康干预中，可以考虑鸢尾素。
