Cao Ying, Jiang Chunhua, Jia Yongsen, Xu Dingjie, Yu Yanyan
College of Traditional Chinese Medicine, North China University of Science and Technology, Tangshan, China.
Reproductive Endocrinology Center, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing, China.
Evid Based Complement Alternat Med. 2020 Jul 20;2020:3687498. doi: 10.1155/2020/3687498. eCollection 2020.
We previously showed that the Chinese herbal medicine, Shaofu Zhuyu decoction (SFZYD), shrank the size of endometriotic lesions in rats with endometriosis. We therefore conducted the present study to investigate the effects of letrozole and SFZYD on gut microbiota in endometriotic rats. Rats were divided into four groups: a blank group, model group, letrozole group, and SFZY group. Ectopic lesion size and COX-2 expression in the endometrium and endometriotic lesions were compared, and the community of gut microbiota was detected using 16S rRNA gene sequencing. Both letrozole and SFZYD reduced the size of ectopic lesions as well as lowered the expression of COX-2, thus reducing the inflammatory response. Compared with the blank group, the -diversity of gut microbiota in endometriotic rats decreased, the ratio increased, and the abundance of was reduced. The -diversity of gut microbiota in the letrozole group was similar to that in the model group, but the ratio was diminished. The -diversity in the SFZY group was similar to that in the blank group, the ratio was attenuated, and the abundance of was elevated compared with the model group. These results indicated that the therapeutic mechanisms of both letrozole and SFZYD were related to the restoration of gut microbiota.
我们之前的研究表明，中药少腹逐瘀汤（SFZYD）可缩小子宫内膜异位症大鼠的异位病灶大小。因此，我们开展了本研究，以探究来曲唑和SFZYD对子宫内膜异位症大鼠肠道微生物群的影响。将大鼠分为四组：空白组、模型组、来曲唑组和SFZY组。比较子宫内膜和异位病灶中异位病灶大小及COX-2表达，并采用16S rRNA基因测序检测肠道微生物群落。来曲唑和SFZYD均减小了异位病灶大小，并降低了COX-2表达，从而减轻了炎症反应。与空白组相比，子宫内膜异位症大鼠肠道微生物群的α多样性降低，β多样性比值增加，且[具体菌属名称]丰度降低。来曲唑组肠道微生物群的α多样性与模型组相似，但β多样性比值减小。SFZY组的α多样性与空白组相似，β多样性比值减弱，且与模型组相比，[具体菌属名称]丰度升高。这些结果表明，来曲唑和SFZYD的治疗机制均与肠道微生物群的恢复有关。
