Odetoyin Babatunde, Adeola Babatunde, Olaniran Olarinde
Department of Medical Microbiology and Parasitology, College of Health Sciences, Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria.
Department of Microbiology, Federal University of Technology, Akure, Ondo State, Nigeria.
J Arthropod Borne Dis. 2020 Mar 31;14(1):88-96. doi: 10.18502/jad.v14i1.2715. eCollection 2020 Mar.
The emergence and spread of antibiotic resistant bacteria has become a serious problem worldwide. Houseflies are potential carriers of pathogenic and resistant bacteria and could be contributing to the global spread of these strains in the environments. We investigated the prevalence and antimicrobial resistant profiles of bacteria isolated from houseflies in Akure.
Twenty-five houseflies were captured by a sterile nylon net from the slaughterhouse, garbage dump, human house, hospital, and eatery from 9:00am to 1:00pm when the flies were active and transported immediately to the laboratory in sterile containers for processing. Bacterial loads were enumerated by serial dilution and plating on nutrient agar and selective media. Bacteria species were isolated by conventional isolation technique. Antibiotic susceptibility test was determined by the Kirby-Bauer disc diffusion technique.
Sixty-seven bacterial species were isolated from 25 samples that were collected. The predominant bacterial species was (n= 31, 45%), followed by (n= 17, 25%), (n= 11, 16%) and (n= 3, 4.3%). The bacterial load of the samples ranged from 9.7×10CFU/mL to 1.65×10CFU/mL The results revealed that all isolates of , spp, and were resistant to streptomycin and cotrimoxazole, augmentin and amoxicillin respectively. None of the isolates was resistant to cotrimoxazole, chloramphenicol, sparfloxacin, augmentin, and ofloxacin. All isolates were multi-drug resistant.
House flies that were collected from the slaughterhouse, garbage dump, human house, hospital, and eatery may participate in the dispersal of pathogenic and resistant bacteria in the study environment.
抗生素耐药菌的出现和传播已成为全球严重问题。家蝇是致病性和耐药菌的潜在携带者，可能促使这些菌株在环境中全球传播。我们调查了从阿库雷的家蝇中分离出的细菌的流行情况和抗菌耐药谱。
上午9点至下午1点家蝇活动时，用无菌尼龙网从屠宰场、垃圾场、民宅、医院和餐馆捕获25只家蝇，并立即将其置于无菌容器中运至实验室进行处理。通过连续稀释并接种于营养琼脂和选择性培养基上计数细菌载量。采用常规分离技术分离细菌种类。通过 Kirby-Bauer 纸片扩散技术进行抗生素敏感性试验。
从采集的25个样本中分离出67种细菌。主要细菌种类为（n = 31，45%），其次是（n = 17，25%）、（n = 11，16%）和（n = 3，4.3%）。样本的细菌载量范围为9.7×10CFU/mL至1.65×10CFU/mL。结果显示，所有分离出的、 spp和分别对链霉素和复方新诺明、奥格门汀和阿莫西林耐药。所有分离出的士均对复方新诺明、氯霉素、司帕沙星、奥格门汀和氧氟沙星不耐药。所有分离株均为多重耐药。
从屠宰场、垃圾场、民宅、医院和餐馆采集的家蝇可能参与了研究环境中致病性和耐药菌的传播。
