诱导多能干细胞作为理解患者对阿片类药物和麻醉剂特异性反应的平台。
Induced pluripotent stem cells as a platform to understand patient-specific responses to opioids and anaesthetics.
作者信息
Obal Detlef, Wu Joseph C
机构信息
Stanford Cardiovascular Institute, Stanford University, Stanford, California, USA.
Department of Anesthesiology, Pain, and Perioperative Medicine, Stanford University, Stanford, California, USA.
出版信息
Br J Pharmacol. 2020 Oct;177(20):4581-4594. doi: 10.1111/bph.15228. Epub 2020 Aug 27.
Abstract
Recent advances in human induced pluripotent stem cell (iPSC) technology may provide unprecedented opportunities to study patient-specific responses to anaesthetics and opioids. In this review, we will (1) examine the advantages and limitations of iPSC technology, (2) summarize studies using iPSCs that have contributed to our current understanding of anaesthetics and opioid action on the cardiovascular system and central nervous system (CNS), and (3) describe how iPSC technology can be used to further develop personalized analgesic and sedative pharmacotherapies with reduced or minimal detrimental cardiovascular effects.
摘要
人类诱导多能干细胞（iPSC）技术的最新进展可能为研究患者对麻醉剂和阿片类药物的特异性反应提供前所未有的机会。在本综述中，我们将：（1）探讨iPSC技术的优势和局限性；（2）总结使用iPSC的研究，这些研究有助于我们目前对麻醉剂和阿片类药物对心血管系统和中枢神经系统（CNS）作用的理解；（3）描述如何利用iPSC技术进一步开发具有减少或最小有害心血管影响的个性化镇痛和镇静药物疗法。
相似文献
1
Induced pluripotent stem cells as a platform to understand patient-specific responses to opioids and anaesthetics.诱导多能干细胞作为理解患者对阿片类药物和麻醉剂特异性反应的平台。
Br J Pharmacol. 2020 Oct;177(20):4581-4594. doi: 10.1111/bph.15228. Epub 2020 Aug 27.
2
Evolution of induced pluripotent stem cell technology.诱导多能干细胞技术的演进。
Curr Opin Hematol. 2010 Jul;17(4):276-80. doi: 10.1097/MOH.0b013e328339f2ee.
3
Induced pluripotent stem cell technology: Toward the future of cardiac arrhythmias.诱导多能干细胞技术：迈向心律失常的未来。
Int J Cardiol. 2017 Jun 15;237:49-52. doi: 10.1016/j.ijcard.2017.03.085. Epub 2017 Mar 21.
4
Central nervous system organoids for modeling neurodegenerative diseases.用于神经退行性疾病建模的中枢神经系统类器官。
IUBMB Life. 2022 Aug;74(8):812-825. doi: 10.1002/iub.2595. Epub 2022 Jan 31.
5
Recent progress of iPSC technology in cardiac diseases.iPSC 技术在心脏疾病中的最新进展。
Arch Toxicol. 2021 Dec;95(12):3633-3650. doi: 10.1007/s00204-021-03172-3. Epub 2021 Oct 17.
6
Structural Changes in N-Glycans on Induced Pluripotent Stem Cells Differentiating Toward Cardiomyocytes.诱导多能干细胞向心肌细胞分化过程中N-聚糖的结构变化
Stem Cells Transl Med. 2015 Nov;4(11):1258-64. doi: 10.5966/sctm.2015-0029. Epub 2015 Sep 16.
7
Human-Induced Pluripotent Stem Cell-Based Differentiation of Cardiomyocyte Subtypes for Drug Discovery and Cell Therapy.基于人诱导多能干细胞的心肌细胞亚型分化用于药物发现和细胞治疗。
Handb Exp Pharmacol. 2023;281:209-233. doi: 10.1007/164_2023_663.
8
Recent advances for using human induced-pluripotent stem cells as pain-in-a-dish models of neuropathic pain.利用人类诱导多能干细胞作为神经病理性疼痛的“盘中模型”的最新进展。
Exp Neurol. 2022 Dec;358:114223. doi: 10.1016/j.expneurol.2022.114223. Epub 2022 Sep 12.
9
Drug discovery using induced pluripotent stem cell models of neurodegenerative and ocular diseases.利用诱导多能干细胞模型进行神经退行性和眼部疾病的药物发现。
Pharmacol Ther. 2017 Sep;177:32-43. doi: 10.1016/j.pharmthera.2017.02.026. Epub 2017 Feb 14.
10
Human Induced Pluripotent Stem Cell (iPSC) Handling Protocols: Maintenance, Expansion, and Cryopreservation.人诱导多能干细胞（iPSC）处理方案：维持、扩增和冷冻保存。
Methods Mol Biol. 2022;2454:1-15. doi: 10.1007/7651_2021_358.
引用本文的文献
1
Investigating the Risk of Arrhythmogenesis Associated With Fentanyl Abuse Using Human and Mouse Cardiomyocytes.利用人和小鼠心肌细胞研究与芬太尼滥用相关的心律失常风险。
Circulation. 2025 Aug 26;152(8):563-566. doi: 10.1161/CIRCULATIONAHA.123.068560. Epub 2025 Aug 25.
2
On the utilization of the induced pluripotent stem cell (iPSC) model to study substance use disorders: A scoping review protocol.利用诱导多能干细胞（iPSC）模型研究物质使用障碍：范围综述方案。
PLoS One. 2023 Oct 12;18(10):e0292238. doi: 10.1371/journal.pone.0292238. eCollection 2023.
3
Adverse Impact of Cannabis on Human Health.大麻对人类健康的不良影响。
Annu Rev Med. 2024 Jan 29;75:353-367. doi: 10.1146/annurev-med-052422-020627. Epub 2023 Aug 15.
4
A human stem cell-derived neuronal model of morphine exposure reflects brain dysregulation in opioid use disorder: Transcriptomic and epigenetic characterization of postmortem-derived iPSC neurons.一种源自人类干细胞的吗啡暴露神经元模型反映了阿片类药物使用障碍中的大脑调节异常：死后来源的诱导多能干细胞神经元的转录组学和表观遗传学特征
Front Psychiatry. 2023 Feb 16;14:1070556. doi: 10.3389/fpsyt.2023.1070556. eCollection 2023.
5
Elucidating effects of environmental exposure using human-induced pluripotent stem cell disease modeling.利用人诱导多能干细胞疾病建模阐明环境暴露的影响。
EMBO Mol Med. 2022 Nov 8;14(11):e13260. doi: 10.15252/emmm.202013260. Epub 2022 Oct 26.
6
Increased Retention of Cardiac Cells to a Glass Substrate through Streptavidin-Biotin Affinity.通过链霉亲和素-生物素亲和力增强心脏细胞对玻璃基质的黏附
ACS Omega. 2021 Jul 1;6(27):17523-17530. doi: 10.1021/acsomega.1c02003. eCollection 2021 Jul 13.
本文引用的文献
1
Kappa but not delta or mu opioid receptors form homodimers at low membrane densities.κ阿片受体而非 δ 或 μ 阿片受体在低膜密度下形成同源二聚体。
Cell Mol Life Sci. 2021 Dec;78(23):7557-7568. doi: 10.1007/s00018-021-03963-y. Epub 2021 Oct 17.
2
Opioids depress breathing through two small brainstem sites.阿片类药物通过脑干的两个小区域抑制呼吸。
Elife. 2020 Feb 19;9:e52694. doi: 10.7554/eLife.52694.
3
Patient and Disease-Specific Induced Pluripotent Stem Cells for Discovery of Personalized Cardiovascular Drugs and Therapeutics.用于发现个体化心血管药物和治疗方法的患者和疾病特异性诱导多能干细胞。
Pharmacol Rev. 2020 Jan;72(1):320-342. doi: 10.1124/pr.116.013003.
4
THE CONCISE GUIDE TO PHARMACOLOGY 2019/20: G protein-coupled receptors.2019/20 年药理学简明指南：G 蛋白偶联受体。
Br J Pharmacol. 2019 Dec;176 Suppl 1(Suppl 1):S21-S141. doi: 10.1111/bph.14748.
5
THE CONCISE GUIDE TO PHARMACOLOGY 2019/20: Ion channels.《药理学 2019/20 简明指南：离子通道》
Br J Pharmacol. 2019 Dec;176 Suppl 1(Suppl 1):S142-S228. doi: 10.1111/bph.14749.
6
Alpha7 nicotinic acetylcholine receptors and neural network synaptic transmission in human induced pluripotent stem cell-derived neurons.α7烟碱型乙酰胆碱受体与人类诱导多能干细胞衍生神经元中的神经网络突触传递
Stem Cell Res. 2019 Dec;41:101642. doi: 10.1016/j.scr.2019.101642. Epub 2019 Oct 31.
7
Engineering of human brain organoids with a functional vascular-like system.人类脑类器官与功能性类血管系统的构建。
Nat Methods. 2019 Nov;16(11):1169-1175. doi: 10.1038/s41592-019-0586-5. Epub 2019 Oct 7.
8
Addiction associated N40D mu-opioid receptor variant modulates synaptic function in human neurons.成瘾相关 N40D μ 阿片受体变异体调节人神经元的突触功能。
Mol Psychiatry. 2020 Jul;25(7):1406-1419. doi: 10.1038/s41380-019-0507-0. Epub 2019 Sep 3.
9
Complex Oscillatory Waves Emerging from Cortical Organoids Model Early Human Brain Network Development.皮质类器官模型中出现的复杂震荡波可模拟早期人类大脑网络发育。
Cell Stem Cell. 2019 Oct 3;25(4):558-569.e7. doi: 10.1016/j.stem.2019.08.002. Epub 2019 Aug 29.
10
State of the clinical science of perioperative brain health: report from the American Society of Anesthesiologists Brain Health Initiative Summit 2018.围手术期脑健康临床科学现状：2018 年美国麻醉医师学会脑健康倡议峰会报告。
Br J Anaesth. 2019 Oct;123(4):464-478. doi: 10.1016/j.bja.2019.07.004. Epub 2019 Aug 19.
Zotero 插件