Pour P M
Eppley Institute for Research in Cancer, University of Nebraska Medical Center, Omaha 68105.
Am J Pathol. 1988 Feb;130(2):335-44.
Electron-microscopic and immunohistochemical studies performed during pancreatic carcinogenesis in hamsters demonstrated that hypertrophy and hyperplasia of centroacinar cells were the earliest changes occurring in the pancreas. These altered centroacinar cells differentiated into either endocrine-type cells or elongated agranular cells with remarkably long cytoplasmic processes (CyPs). These CyPs seemed gradually to overlie and underlie the adjacent acinar cells and resulted in progressive degeneration and loss of acinar cells, which subsequently were replaced by altered centroacinar cells. The initially rather tiny and slender CyPs were characterized by the expression of blood group substances, which were also found in the surface of altered ductal cells. Because these antigens could not be demonstrated in normal pancreatic cells, they seemed to represent specific markers for altered ductal/ductular (centroacinar) cells. In no instance was there evidence of dedifferentiation of acinar cells into ductlike cells. The present data, along with our previous findings, demonstrate that centroacinar cells are the foundation for pseudoductular structures and are the progenitor cells of tumors arising from them.
在仓鼠胰腺癌发生过程中进行的电子显微镜和免疫组织化学研究表明，腺泡中心细胞的肥大和增生是胰腺中最早出现的变化。这些改变的腺泡中心细胞分化为内分泌型细胞或具有明显长细胞质突起（CyPs）的细长无颗粒细胞。这些CyPs似乎逐渐覆盖和位于相邻腺泡细胞的上方和下方，导致腺泡细胞逐渐退化和丧失，随后被改变的腺泡中心细胞取代。最初相当微小和细长的CyPs的特征是表达血型物质，在改变的导管细胞表面也发现了这些物质。因为这些抗原在正常胰腺细胞中无法显示，它们似乎代表了改变的导管/小导管（腺泡中心）细胞的特异性标志物。在任何情况下都没有腺泡细胞去分化为导管样细胞的证据。目前的数据以及我们之前的发现表明，腺泡中心细胞是假导管结构的基础，并且是由它们产生的肿瘤的祖细胞。
