Liu Kangsheng, Tong Hua, Li Taiping, Chen Yajun, Mao Xiaodong
Department of Clinical Laboratory, Women's Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital Nanjing 210029, Jiangsu, China.
Department of Obstetrics and Gynecology, Women's Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital Nanjing 210029, Jiangsu, China.
Am J Transl Res. 2020 Jul 15;12(7):3225-3233. eCollection 2020.
Though the survival of patients with gynecological tumors has been significantly prolonged by radiotherapy, chemotherapy, targeted therapy and other treatments, the way to improve the patients' life quality still needs investigation. Circulating tumor DNA (ctDNA), which contains tumor genetic information, has the potential in early diagnosis of malignancies due to its high consistency with tumor tissues. Using the key words including "digital PCR", "ctDNA", "technology of digital PCR", and "detection method", "gynecological tumor", we retrieved the original articles and reviews in PubMed and WEB OF SCI database published before May 10, 2019 and performed a thorough review of them. The analysis of ctDNA could provide a comprehensive description of tumor genome, overcome the heterogeneity of tissue biopsy, and supplement the missing mutations in tissue samples. Furthermore, ctDNA could be used as a target of liquid biopsy. Our study also showed that digital PCR technology has a good potential to detect ctDNA in gynecological tumors.
尽管放疗、化疗、靶向治疗等手段显著延长了妇科肿瘤患者的生存期，但提高患者生活质量的方法仍有待探索。循环肿瘤DNA（ctDNA）包含肿瘤遗传信息，由于其与肿瘤组织高度一致，在恶性肿瘤的早期诊断中具有潜力。我们使用“数字PCR”“ctDNA”“数字PCR技术”“检测方法”“妇科肿瘤”等关键词，检索了2019年5月10日前发表在PubMed和WEB OF SCI数据库中的原始文章及综述，并对其进行了全面综述。对ctDNA的分析可以全面描述肿瘤基因组，克服组织活检的异质性，并补充组织样本中缺失的突变。此外，ctDNA可作为液体活检的靶点。我们的研究还表明，数字PCR技术在检测妇科肿瘤中的ctDNA方面具有良好潜力。
