Guo Feng, Li Shanqing, Guo Chao, Xu Xiaohui, Zhou Xiaoyun, Ma Dongjie, Cao Zhili, Bing Zhongxing, Cui Yushang
Department of Thoracic Surgery, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences Beijing 100730, China.
Am J Transl Res. 2020 Jul 15;12(7):3953-3963. eCollection 2020.
The emerging roles of circular RNAs (circRNAs) in non-small cell lung cancer (NSCLC) have been convincingly proved. However, there are still numerous unknown circRNAs needing exploration. Here, present research performed a circRNA microarray analysis for the expression profile and identified a novel circRNA (circMAGI3, hsa_circ_0110498). Clinically, circMAGI3 was significantly up-regulated in NSCLC tissue and cells, which was closely correlated with unfavorable outcome for NSCLC patients. Functionally, circMAGI3 promoted the glycolysis and proliferation of NSCLC cells. Mechanistically, circMAGI3 functioned as a sponge for miR-515-5p to relieve its target gene HDGF expression, thereby accelerating the glycolysis of NSCLC. Collectively, this research identified the oncogenic role of circMAGI3 in the tumorigenesis through miR-515-5p/HDGF axis, providing a vital theoretical basis for treatment of NSCLC.
环状RNA（circRNAs）在非小细胞肺癌（NSCLC）中的新作用已得到令人信服的证实。然而，仍有许多未知的circRNAs有待探索。在此，本研究对表达谱进行了circRNA微阵列分析，并鉴定出一种新型circRNA（circMAGI3，hsa_circ_0110498）。在临床上，circMAGI3在NSCLC组织和细胞中显著上调，这与NSCLC患者的不良预后密切相关。在功能上，circMAGI3促进NSCLC细胞的糖酵解和增殖。机制上，circMAGI3作为miR-515-5p的海绵，以减轻其靶基因HDGF的表达，从而加速NSCLC的糖酵解。总的来说，本研究通过miR-515-5p/HDGF轴确定了circMAGI3在肿瘤发生中的致癌作用，为NSCLC的治疗提供了重要的理论基础。
